We narrowed to 9,756 results for: Gnas

1. pcDNA3.1-Hygro(+)-mscGAS

   Plasmid

   #102607

   Purpose

   Mammalian expression of mouse cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   cGAS (Cgas Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-GFP11-Actin

   Plasmid

   #181966

   Purpose

   Human beta actin tagged with GFP11.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11-Actin (ACTB Human)
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-DIO-EF1α-mKate2-biPAC

   Plasmid

   #169127

   Purpose

   AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Insert

   mKate2-biPAC
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   codon optimized to mouse

   Promoter

   EF1a

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-P3-IgKL-mNG

   Plasmid

   #183465

   Purpose

   To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   IgKL-mNeonGreen
   

   Use

   AAV

   Tags

   IgKL secretory signal

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-GFP11(x7)-Actin

   Plasmid

   #181967

   Purpose

   Human beta actin tagged with 7 copies of GFP11.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11(x7)-Actin (ACTB )
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-CMV-mCherry-hPMCA2w/b

   Plasmid

   #111570

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GAL-p300-230-670

   Plasmid

   #89099

   Purpose

   mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 230-670

   Promoter

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GAL-p300-1601-1900

   Plasmid

   #89102

   Purpose

   mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 1601-1900

   Promoter

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GAL-p300-501-999

   Plasmid

   #89100

   Purpose

   mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 501-999

   Promoter

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBSFI-myr-akt-T308D/S473D

    Plasmid

    #49192

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308D; S473D

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLJM1-TMEM192-FLAG

    Plasmid

    #164977

    Purpose

    Lentiviral vector for TMEM192 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTBRII / CEP-Zeo / Hygro

    Plasmid

    #16622

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Insert

    TGF beta type II receptor (TGFBR2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pTBK1

    Plasmid

    #87443

    Purpose

    express TBK1 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    TBK1 (Tbk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-PKAcat-mCherry

    Plasmid

    #181841

    Purpose

    mCherry-tagged PKA catalytic subunit for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PKAcat-mCherry (PRKACA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pH1R-mCherry-N1

    Plasmid

    #84325

    Purpose

    Histamine-1 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R (HRH1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-neo-CD2-FAK

    Plasmid

    #37013

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    CD2-FAK (CD2, PTK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NES-mCherry-cPHx3

    Plasmid

    #116862

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SBP-GFP-Tac-TC

    Plasmid

    #162506

    Purpose

    Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    

    Use

    Tags

    GFP and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP Abi1

    Plasmid

    #74905

    Purpose

    Expresses human Abi1 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

    Insert

    Abi1 (ABL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-Epac1 CAAX

    Plasmid

    #118312

    Purpose

    Expression of human Epac1

    Depositor

    Philip Stork

    Article

    Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

    Insert

    Epac1 CAAX (RAPGEF3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal 20aa of KiRas

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 P-cad

    Plasmid

    #47502

    Purpose

    Expresses P-cadherin in mammalian cells

    Depositor

    Alice Wong

    Article

    Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

    Insert

    cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3-HA PSPC1

    Plasmid

    #101764

    Purpose

    Expresses PSPC1 in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    paraspeckle component 1 (PSPC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO-CreERT2 sgRipk4 v3

    Plasmid

    #158048

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pLKO-CreERT2 sgAdam10 v3

    Plasmid

    #158047

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. GFP-Epac1 SAAX

    Plasmid

    #118313

    Purpose

    Expression of human Epac1

    Depositor

    Philip Stork

    Article

    Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

    Insert

    Epac1 SAAX (RAPGEF3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPC-Flag-SOCS1

    Plasmid

    #129514

    Purpose

    Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX6P1-HA-MEK1

    Plasmid

    #53159

    Purpose

    Expresses GST-HA tagged human MEK1 from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LentiCRISPRv2 Blast CRLF2

    Plasmid

    #194090

    Purpose

    Expression of human CRLF2 gene

    Depositor

    Martin Carroll

    Article

    Hurtz et al J Clin Invest. 2020 Jul 1;130(7):3637-36

    Insert

    CRLF2 (CRLF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLL5.0-MRLC-DD-GFP

    Plasmid

    #187260

    Purpose

    Lentiviral expression of myosin regulatory light chain-DD mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18D, S19D

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLL5.0-MRLC-AA-GFP

    Plasmid

    #187259

    Purpose

    Lentiviral expression of myosin regulatory light chain-AA mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18A,S19A

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 3XFLAG_GAPDH in pCW57_tTA_Blast

    Plasmid

    #154211

    Purpose

    Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE repeats (dox off)

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-AHPH-DM

    Plasmid

    #71368

    Purpose

    Location sensor for RhoA-GTP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    C terminus of Anillin (ANLN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A740D and E758K

    Promoter

    CMV

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA 3.3 HA-hTSPAN12

    Plasmid

    #115784

    Purpose

    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    TSPAN12 (TSPAN12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    multiple silent mutations introduce restriction s…

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5-FLAG-SLC38A9.1

    Plasmid

    #71855

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-BCR-ABL-p190-FLAG

    Plasmid

    #205620

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p190 isoform b3a2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T117M- Please see depositor comments

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pH1R-P2A-mCherry-N1

    Plasmid

    #84330

    Purpose

    Co-expresses (untagged) Histamine-1 receptor and mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R-P2A-mCherry (HRH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. POLD1_pLX307

    Plasmid

    #98358

    Purpose

    Lentiviral expression of POLD1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GAL-p300-1000-1600

    Plasmid

    #89101

    Purpose

    mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1000-1600

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GAL-p300-2-300

    Plasmid

    #89098

    Purpose

    mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 2-300

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. GAL-p300-1901-2414

    Plasmid

    #89103

    Purpose

    mammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1901-2414

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEF5/FRT/DEST-3xHA/mGli2

    Plasmid

    #51250

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

47. GfaABC1D BARK D110A p2A mCherry

    Plasmid

    #117692

    Purpose

    GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Use

    AAV

    Tags

    HA / FLAG

    Expression

    Mutation

    D110A

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLVX-CMV-Galpha-z ONE-GO

    Plasmid

    #189734

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-z ONE-GO
    GNAZ (Gnaz Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pCMV-hARdeltaNH2-6

    Plasmid

    #89104

    Purpose

    mammalian expression of human androgen receptor amino acids 507-919

    Depositor

    Elizabeth Wilson

    Article

    Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

    Insert

    hAR DNA binding domain+ligand binding domain (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NH2 terminal deletion, contains amino acids 507-…

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pmCherry-HP1alpha

    Plasmid

    #179703

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge