4,176 results

1. OKSIM

   Plasmid

   #24603

   Depositor

   Jose Cibelli

   Article

   Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

   Insert

   Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   Oct4-2A-KLF4-2A-Sox2-IRES-Myc

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-Bcl2

   Plasmid

   #18003

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   Bcl-2 (BCL2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-c-Myc

   Plasmid

   #13375

   Purpose

   Retroviral expression of mouse c-Myc

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_GPHN.FingR-mKate2-IL2RGTC

   Plasmid

   #46297

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mNeptune2.5

   Plasmid

   #51310

   Purpose

   Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2.5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. paavCAG-pre-mGRASP-mCerulean

   Plasmid

   #34910

   Purpose

   pre

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Has Service

   AAV5

   Insert

   presynaptic mGRASP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCerulean3-N1

   Plasmid

   #54730

   Purpose

   Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Type

   Empty backbone

   Tags

   mCerulean3

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIEG3-c-Jun

   Plasmid

   #40348

   Depositor

   Alexander Dent

   Article

   Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

   Insert

   c-Jun (JUN Human)
   

   Use

   Retroviral

   Tags

   IRES eGFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLOX-TERT-iresTK

    Plasmid

    #12245

    Purpose

    2nd generation lentiviral vector for reversible immortalization of human primary cells

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    TERT, HSV1-TK (TERT Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST/hCx43-EGFP-N1

    Plasmid

    #40907

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    Connexin 43 (GJA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-SIRT1 H363Y

    Plasmid

    #1792

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H363Y, deacetylase domain mutation

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA (New MCS)

    Plasmid

    #32530

    Depositor

    Christopher A Walsh

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TLR8-YFP

    Plasmid

    #13024

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 8 (TLR8 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK232 (CMV-OsTIR1-PURO)

    Plasmid

    #72834

    Purpose

    Expresses OsTIR1 under the control of the CMV promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-CMV-OsTIR1-PURO-AAVS1
    

    Expression

    Mammalian

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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