19,627 results

1. p(+)MV-NSE-FlagP-M502-3p

   Plasmid

   #58748

   Purpose

   Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence

   Depositor

   Branka Horvat

   Insert

   Measles virus antigenome
   

   Tags

   Flag

   Promoter

   T7

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-Flag-CtIP-T847E

   Plasmid

   #71110

   Purpose

   mammalian expession of Flag-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pSBbi-Pur

   Plasmid

   #60523

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. TrkB Acceptor

   Plasmid

   #83953

   Purpose

   FRET acceptor for TrkB mEGFP

   Depositor

   Ryohei Yasuda

   Article

   Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

   Insert

   Phospholipase C gamma 1 (Plcg1 Mouse)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-NFAT luciferase

   Plasmid

   #17870

   Purpose

   3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

   Depositor

   Jerry Crabtree

   Article

   Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

   Insert

   3x NFAT binding sequence (IL2 )
   

   Use

   Luciferase

   Tags

   IL2 promoter and Luciferase

   Expression

   Mammalian

   Promoter

   IL-2 promoter

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-PGK-ABI-dCas9-P2A-mcherry

   Plasmid

   #121513

   Purpose

   expression of AB1-fused-dcas9 plasmids

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   AB1 and P2A-mcherry

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FGFR2 3xFlag

   Plasmid

   #110063

   Purpose

   FGFR2 expression in mammalian cells (JCOPCO paper)

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Tags

   Flag tag with linker and mitochondrial targeting …

   Mutation

   Human codon optimized

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1 pegRNA1 with trimmed attP

   Plasmid

   #222346

   Purpose

   pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA1
   

   Use

   CRISPR

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9-2A-Cre

    Plasmid

    #68468

    Purpose

    co-expressing Cas9 and Cre recombinase

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    Cas9 and Cre recombinase
    

    Use

    CRISPR

    Tags

    P2A-Cre-Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_PGK_antiCD19_synNotch_TetRVP64

    Plasmid

    #79126

    Purpose

    Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    antiCD19_synNotch_TetRVP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGK-DTAG-CTERM-GFP

    Plasmid

    #185770

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAPB02

    Plasmid

    #65628

    Purpose

    A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

    Depositor

    Pei-yuan Qian

    Article

    Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAX APP 751

    Plasmid

    #30138

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPLICS Po-ER Short P2A

    Plasmid

    #164114

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-BTK-mCherry

    Plasmid

    #50043

    Purpose

    Expression of BTK-mCherry in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BTK (Btk Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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