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174,739 results

Showing: 4241 - 4280 of 174739 results

  10. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  14. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

    Plasmid
    #103864
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103864-plasmid-map-sequence-id-199176

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  15. pSNAPf-H2B Control Plasmid

    Plasmid
    #101124
    Purpose
    Expression of SNAP-tagged Histone H2B in mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    histone cluster 1 H2B family member j (H2BC11 Human)
    Tags
    SNAP-tag (SNAPf)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101124-plasmid-map-sequence-id-194083

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  16. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  20. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    81029-plasmid-map-sequence-id-300392

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  25. pSBtet-Bla

    Plasmid
    #60510
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60510-plasmid-map-sequence-id-97536

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  26. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  28. mCherry-Vinculin-23

    Plasmid
    #55159
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55159-plasmid-map-sequence-id-220874

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  29. mTurquoise2-pBAD

    Plasmid
    #54844
    Purpose
    Localization: Protein Expression Vector , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6x His, T7 tag, and TEV site
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54844-plasmid-map-sequence-id-139002

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  37. pmEGFP-1

    Plasmid
    #36409
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Fluorescent protein cassette for sub-cloning
    Tags
    mEGFP
    Promoter
    no promoter
    Available Since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    36409-plasmid-map-sequence-id-45554

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Showing: 4241 - 4280 of 174739 results