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163,687 results

Showing: 5301 - 5350 of 163687 results

  1. pCAG2-EGFP-C-IFT144

    Plasmid
    #218740
    Purpose
    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT144 (WDR19 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218740-plasmid-map-sequence-id-434235

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  6. mCherry-Lifeact-7

    Plasmid
    #54491
    Purpose
    Localization: Action, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mCherry-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54491-plasmid-map-sequence-id-227430

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  9. pcDNA3.1-hSyncytin-1

    Plasmid
    #182440
    Purpose
    hSyncytin-1
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182440-plasmid-map-sequence-id-357693

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  10. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

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  11. pCNA

    Plasmid
    #135191
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-CreI lamba Red
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135191-plasmid-map-sequence-id-273788

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  15. pSNK

    Plasmid
    #135193
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135193-plasmid-map-sequence-id-288121

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  18. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  22. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid
    #61593
    Purpose
    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
    Depositor
    Feng Zhang
    Article
    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    HA, NLS, and OLLAS tag
    Expression
    Mammalian
    Mutation
    K175R and K736R (deUb mutations)
    Promoter
    Available Since
    Feb. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61593-plasmid-map-sequence-id-470051

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  23. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  24. pASPIre2

    Plasmid
    #154843
    Purpose
    Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154843-plasmid-map-sequence-id-307634

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  32. pSNA

    Plasmid
    #135192
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135192-plasmid-map-sequence-id-296242

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  38. TFORF1907

    Plasmid
    #141884
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF7L2 (TCF7L2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141884-plasmid-map-sequence-id-415060

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  43. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid
    #29655
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, GST, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29655-plasmid-map-sequence-id-196920

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Showing: 5301 - 5350 of 163687 results