We narrowed to 10,767 results for: Kars

1. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a_MYC deltaCTD_P2A_Hygro_Barcode

   Plasmid

   #120511

   Purpose

   Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaCTD (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of carboxy-terminal domain

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF1a_MYC deltaNTD_P2A_Hygro_Barcode

   Plasmid

   #120510

   Purpose

   Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaNTD (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of amino-terminal domain

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EF1a_MYC deltaMBI_P2A_Hygro_Barcode

   Plasmid

   #120504

   Purpose

   Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaMBI (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of MYC Box I domain

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EF1a_MYC deltab_P2A_Hygro_Barcode

   Plasmid

   #120507

   Purpose

   Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltab (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of basic motif

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

   Plasmid

   #120505

   Purpose

   Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   c-MYC deltaMBII (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of MYC Box II domain

   Promoter

   EF1a

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-PPP2R1A

   Plasmid

   #116777

   Purpose

   Lentiviral expression of PPP2R1A

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PPP2R1A (PPP2R1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-XPO1

   Plasmid

   #116804

   Purpose

   Lentiviral expression of XPO1

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   XPO1 (XPO1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SPHKAP scFv [L131/17]

   Plasmid

   #190511

   Purpose

   Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_MYC deltaLZ_P2A_Hygro_Barcode

    Plasmid

    #120509

    Purpose

    Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaLZ (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of leucine zipper motif

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

    Plasmid

    #120508

    Purpose

    Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaHLH (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of helix-loop-helix motif

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF1a_KLF6_P2A_Hygro_Barcode

    Plasmid

    #120492

    Purpose

    Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF6 (KLF6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLIB_GST-TEV-SRC (Y530F)

    Plasmid

    #223743

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Src Kinase (SRC Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Y530F constitutively active mutant

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

    Plasmid

    #216320

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice donor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-ROR1

    Plasmid

    #116789

    Purpose

    Lentiviral expression of ROR1

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ROR1 (ROR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VGlut1 scFv [N28/9]

    Plasmid

    #182084

    Purpose

    Mammalian Expression of VGlut1 scFv. Derived from hybridoma N28/9.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting VGlut1 (Rattus norvegicus) (Slc17a7 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pALS2-sfGFP 150TAG

    Plasmid

    #197574

    Purpose

    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NINJA TC

    Plasmid

    #159274

    Purpose

    Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

    Depositor

    Nikhil Joshi

    Article

    Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    NINJA TC
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRES2-EGFP-deltaNp53:WTp53

    Plasmid

    #49244

    Purpose

    expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN and p53 WT (TP53 Human)
    

    Use

    Tags

    His and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-PTCH1

    Plasmid

    #116779

    Purpose

    Lentiviral expression of PTCH1

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTCH1 (PTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE-SRSF2

    Plasmid

    #116793

    Purpose

    Lentiviral expression of SRSF2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas-MmdCas12m-CBE1

    Plasmid

    #192284

    Purpose

    MmdCas12m-CBE1 expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m-CDA-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

    Plasmid

    #176085

    Purpose

    EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    BRCT2 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

    Plasmid

    #176074

    Purpose

    EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    BRCT1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pan-Synapsin scFv [L125/129]

    Plasmid

    #182079

    Purpose

    Mammalian Expression of pan-Synapsin scFv. Derived from hybridoma L125/129.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting pan-Synapsin
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-DDX43

    Plasmid

    #134574

    Purpose

    Express human DDX43 protein (WT, full-length) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43 (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length WT

    Promoter

    T7

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EF1a_HNF4A_P2A_Hygro_Barcode

    Plasmid

    #120450

    Purpose

    Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG_GST-TEV-EGFP-ATG13

    Plasmid

    #223797

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens, James Hurley

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST-TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-STK19

    Plasmid

    #116795

    Purpose

    Lentiviral expression of STK19

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    STK19 (STK19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EF1a_HNF1A_P2A_Hygro_Barcode

    Plasmid

    #120448

    Purpose

    Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6 and U6

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 6-His-MBP-TEV-FnCpf1

    Plasmid

    #90094

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 (humanized)
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CPSF4

    Plasmid

    #155477

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE-STK11

    Plasmid

    #116794

    Purpose

    Lentiviral expression of STK11

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    STK11 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHAGE-MRPS31

    Plasmid

    #116764

    Purpose

    Lentiviral expression of MRPS31

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MRPS31 (MRPS31 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223 SARS-CoV-2 ORF3B

    Plasmid

    #141272

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid

    #154196

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC1-A, sgRNA-BC1-C
    

    Use

    Lentiviral; Catch barcode activation

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and mU6

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. MYC-PGK-blast

    Plasmid

    #190618

    Purpose

    Lentiviral expression plasmid of human MYC, blast selection

    Depositor

    Junwei Shi

    Article

    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. THOC4

    Plasmid

    #156083

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC4 (ALYREF Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1 His-GFP-EWSR1-myc-His

    Plasmid

    #46385

    Purpose

    expresses EGFP tagged EWSR1 in mammalian cells

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    EGFP, His, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

44. CPSF4-1

    Plasmid

    #155478

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EF1a_RUNX1_P2A_Hygro_Barcode

    Plasmid

    #120475

    Purpose

    Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid

    #154197

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC2-A, sgRNA-BC2-C
    

    Use

    Lentiviral; Catch barcode activation

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and mU6

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

    Plasmid

    #216319

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cerulean + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

    Plasmid

    #216318

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    split cerulean fluorescent protein + splice donor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. EXOSC10

    Plasmid

    #155589

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMXs-hM₃O-IP

    Plasmid

    #46645

    Depositor

    Nobuaki Kikyo

    Article

    Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

    Insert

    hM₃O (POU5F1 Human)
    

    Use

    Retroviral

    Tags

    IRES Puro and human MyoD (aa 1-62)

    Expression

    Mammalian

    Mutation

    contains a fragment of human MyoD (aa1-62) fused …

    Promoter

    Available since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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