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We narrowed to 5,937 results for: plasmid dna

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Showing: 5351 - 5400 of 5937 results

  1. pcPylRS(Y384F Y306A)

    Plasmid
    #196486
    Purpose
    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    196486-plasmid-map-sequence-id-386704

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  4. MmXYRS-4xM15

    Plasmid
    #155343
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    Humanized MmXYRS/tRNAM15
    Expression
    Mammalian
    Promoter
    CMV/U6
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155343-plasmid-map-sequence-id-310521

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  5. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  10. pCK300

    Plasmid
    #87766
    Purpose
    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87766-plasmid-map-sequence-id-177280

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  11. 1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

    Plasmid
    #238894
    Purpose
    EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.
    Depositor
    Gil Westmeyer
    Article
    Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4
    Insert
    1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    238894-plasmid-map-sequence-id-476197

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  18. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Tags
    Met-6xHis
    Expression
    Bacterial
    Promoter
    T5 promoter
    Available Since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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  19. TEV-HRV-3C Proteases-mCherry

    Plasmid
    #243795
    Purpose
    Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TEV and HRV-3C proteases
    Expression
    Mammalian
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243795-plasmid-map-sequence-id-486454

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  27. TVMV-HRV-3C Proteases-mCherry

    Plasmid
    #243796
    Purpose
    Encodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TVMV and HRV-3C proteases
    Expression
    Mammalian
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    243796-plasmid-map-sequence-id-489527

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  28. TVMV-TEV Proteases-mCherry

    Plasmid
    #243797
    Purpose
    Encodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    TVMV and TEV proteases
    Expression
    Mammalian
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243797-plasmid-map-sequence-id-486448

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  29. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

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  30. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  34. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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  35. MBP-g0s2

    Plasmid
    #179294
    Purpose
    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    mature maltose binding protein + enterokinase cle…
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    Ptac
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179294-plasmid-map-sequence-id-354917

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  49. pHrD-IRES-Fluc

    Plasmid
    #194250
    Purpose
    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!
    Depositor
    Samson Jacob
    Article
    McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do
    Insert
    Firefly luciferase
    Use
    Luciferase
    Promoter
    human rDNA promoter and beginning of 5' ETS …
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    194250-plasmid-map-sequence-id-391338

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  50. pMTMVi-M

    Plasmid
    #118757
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Available Since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    118757-plasmid-map-sequence-id-231795

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Showing: 5351 - 5400 of 5937 results