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20,112 results

Showing: 3241 - 3270 of 20112 results

  11. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  13. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  16. pLEX_306

    Plasmid
    #41391
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41391-plasmid-map-sequence-id-473523

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  23. pSBtet-RH

    Plasmid
    #60500
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60500-plasmid-map-sequence-id-97547

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  24. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-TET
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

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Showing: 3241 - 3270 of 20112 results