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Plasmid#205305PurposeExpression of WHaloCaMP1b-EGFP fusion in E. coliDepositorInsertWHaloCaMP1b-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS2-blast
Plasmid#158458PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TrpRS-His
Plasmid#124120PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTryptophanyl-tRNA ligase (trpS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSetB-KanR-LiLac
Plasmid#184569PurposeFluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expressionDepositorInsertLiLac fluorescent sensor of lactate
UseTagsHis7ExpressionBacterialMutationPromoterT7Available sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETB PKA RIalpha
Plasmid#14922DepositorInsertPKA R1alpha regulatory subunit (PRKAR1A Bovine)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-V5_pRS415
Plasmid#199667Purposeexpresses LOV-Turbo in the yeast cytosolDepositorInsertLOV-Turbo
UseTagsV5ExpressionYeastMutationPromoterGal1,10Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET iGluSnFR3 v857
Plasmid#175186PurposeFluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.DepositorInsertiGluSnFR3 v857
UseSynthetic BiologyTagsHis tag, T7 leader, Xpress epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405-FlagPEN2/PS1wt
Plasmid#207803PurposeYeast integrative vector for expression of FlagPEN2 and Presenilin 1DepositorInsertGamma-secretase subunits PEN-2 and presenilin1 wt
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-Etanercept
Plasmid#182420PurposeBacterial expression of mNeongreen-Etanercept fusionDepositorInsertmNeongreen-Etanercept (TNFRSF1B Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorInsertMB21D1 (CGAS Human)
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_AnapRS
Plasmid#140019PurposePlasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integrationDepositorInsertEcoLeuRS (AnapRS)
UseTagsExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD-SepRS-EFSep
Plasmid#34623DepositorUseTagsExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtac and Ptac (from SepRS- operon like)Available sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS3 (CK2alpha, CK2beta)
Plasmid#27092DepositorUseTetracycline-regulated expressionTagsHA and MycExpressionMammalianMutationPromoterbidirectional tet-responsive promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-sgRNA(siteA)
Plasmid#154831PurposesgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cellsDepositorInsertsgRNA(siteA)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJ1806 pRS413-COF1RFP
Plasmid#37101DepositorInsertCOF1-RFP
UseTagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS2-puro
Plasmid#158457PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-mCherry
Plasmid#113552PurposeFor bacterial expression and purification of mCherryDepositorInserthis-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS-puro shNEDD4L #1
Plasmid#27016DepositorInsertshRNA NEDD4L #1 (NEDD4L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-strongSC_GFP
Plasmid#22316DepositorInsertGFP-rad9-GFP
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-His6
Plasmid#182386PurposeBacterial expression of Vesicle Nucleating peptideDepositorInsertVNp-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-pLtetO-gfp
Plasmid#89476PurposeBroad-host plasmid expressing gfp under pLtetO promoterDepositorInsertpLtetO-GFPmut3b
UseTagsExpressionMutationPromoterpLtetOAvailable sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS405-PTEF-Dcr1
Plasmid#22314DepositorInsertDCR1
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS404-PTEF-Ago1
Plasmid#22313DepositorInsertAGO1
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(D10A)
Plasmid#79618PurposeExpresses nCas9(D10A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-v2 RsTAL At4CL
Plasmid#126524Purposeexpresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesisDepositorInsertsUseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorInsertG4G6 (GSN Human)
UseTags6xHis-MBPExpressionBacterialMutationWTPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R12
Plasmid#83311PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R12
UseTags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/N149K/F223R/S202KPromoterT7Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTEF1-yEGFPCLN2PEST-pRS406
Plasmid#64406PurposeTEF promoter driven GFP expressionDepositorInsertTEF1 promoter (TEF1 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-FBXL5-SKP1
Plasmid#226620PurposeCo-expression of FBXL5-SKP1 complex in E.coliDepositorUseTagsGB1ExpressionBacterialMutationAmino acids 1-198 was deleted, and 420-596 was re…PromoterT7Available sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-eGFP
Plasmid#113551PurposeFor bacterial expression and purification of eGFPDepositorInserthis-eGFP
UseTagsExpressionBacterialMutationA206KPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-His-APEX2
Plasmid#201471PurposeRecombinant soluble APEX2 for SLAPSHOT labelingDepositorInsertAPEX2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS11D-G418
Plasmid#158477PurposeConstitutive lentiviral expression of human TMPRSS11D.DepositorInsertTMPRSS11D (TMPRSS11D Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS11D-blast
Plasmid#158476PurposeConstitutive lentiviral expression of human TMPRSS11D.DepositorInsertTMPRSS11D (TMPRSS11D Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET iGluSnFR3 v82
Plasmid#175187PurposeFluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.DepositorInsertiGluSnFR3 v82
UseSynthetic BiologyTagsHis tag, T7 leader, Xpress epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-600n
Plasmid#13537DepositorInsertFLIP-E WT
UseTagsECFP and VenusExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS303-PADH1-AFB2
Plasmid#99530PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)DepositorInsertAFB2 (AFB2 Mustard Weed)
UseTagsExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pscALPSblasti-TMPRSS2 Blasti
Plasmid#158088PurposeExpresses TMPRSS2 in mammalian cellsDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-DARPinOFF7
Plasmid#182398PurposeBacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusionDepositorInsertVNp6-DARPinOFF7
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-XK
Plasmid#184369PurposeExpress human XK in Expi293 cellsDepositorInsertHuman XK (XK Human)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416Gal TDP43 WT YFP
Plasmid#27447DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS424GFP-FYVE(EEA1)
Plasmid#36096DepositorInsertFYVE(EEA1) domain (EEA1 Human)
UseTagsGFPExpressionYeastMutationPromoterPRC1Available sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRST7-ER beta (long)
Plasmid#11356DepositorInsertER beta long (ESR2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
UseTagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSB19-PapRS#34-OmpA
Plasmid#231105PurposeVector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSET.GltI253-cpGFP.L1LV/L2NP
Plasmid#41733Purposea single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expressionDepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterT7Available sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only