Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 13,963 results for: eng

Clear keywords
Showing: 201 - 220 of 13963 results

  4. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145145-plasmid-map-sequence-id-324821

    Enlarge

  5. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172878-plasmid-map-sequence-id-340526

    Enlarge

  6. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40132-plasmid-map-sequence-id-51020

    Enlarge

  10. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid
    #40131
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (KKR)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40131-plasmid-map-sequence-id-51019

    Enlarge

  14. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

    Enlarge

  16. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

    Enlarge

Showing: 201 - 220 of 13963 results