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179,521 results

Showing: 4361 - 4400 of 179521 results

  14. CMV-Hylight

    Plasmid
    #193447
    Purpose
    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)
    Depositor
    Richard Goodman
    Publication
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hylight Biosensor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    193447-plasmid-map-sequence-id-378450

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  15. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Publication
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  16. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid
    #171588
    Purpose
    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
    Depositor
    Kenneth Matreyek
    Publication
    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.
    Inserts
    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171588-plasmid-map-sequence-id-335078

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  17. pCW57.1 CMYC

    Plasmid
    #164145
    Purpose
    Doxycycline inducible expression of c-MYC
    Depositor
    Richard Possemato
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Doxycycline inducible promoter
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164145-plasmid-map-sequence-id-325859

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  20. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138148-plasmid-map-sequence-id-318593

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  24. pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro

    Plasmid
    #141380
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp14 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141380-plasmid-map-sequence-id-288093

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  25. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

    Plasmid
    #141387
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141387-plasmid-map-sequence-id-288074

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  27. pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

    Plasmid
    #141369
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141369-plasmid-map-sequence-id-288061

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  33. anxA2-GFP

    Plasmid
    #107196
    Purpose
    Expresses human annexin A2-GFP fusion protein for live cell imaging
    Depositor
    Volker Gerke, Ursula Rescher
    Publication
    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    annexin A2 (ANXA2 Human)
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    Ala residue 65 in human sequence replaced by Glu …
    Promoter
    CMV
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107196-plasmid-map-sequence-id-209665

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  35. pC0052-REPAIR non-targeting guide clone into pC0043

    Plasmid
    #103868
    Purpose
    Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    non-targeting guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103868-plasmid-map-sequence-id-199182

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  36. pGL4-UPRE-luc2P-Hygro

    Plasmid
    #101788
    Purpose
    Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)
    Depositor
    Seiichi Oyadomari
    Publication
    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xUPRE
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101788-plasmid-map-sequence-id-194141

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  39. pLV-Golgi eGFP

    Plasmid
    #79809
    Purpose
    To express and target GFP into the Golgi apparatus using lentiviruses
    Depositor
    Pantelis Tsoulfas
    Insert
    Golgi eGFP (B4GALT1 Human)
    Use
    Lentiviral
    Tags
    Fusion of the first 62 amino acids of the human b…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79809-plasmid-map-sequence-id-500311

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Showing: 4361 - 4400 of 179521 results