163,606 results

1. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227898

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227899

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRcCMV myc-Plk1 T210D (Nigg LA183)

   Plasmid

   #41158

   Depositor

   Erich Nigg

   Article

   Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

   Insert

   Plk1 (PLK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T210D

   Promoter

   CMV

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227890

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5xATF6-GL3

   Plasmid

   #11976

   Purpose

   Reporter gene with five ATF6 sites that is highly sensitive to ER stress

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   5xATF6 binding site
   

   Use

   Tags

   Luc and c-fos promoter

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCG-TPMV Fd32

   Plasmid

   #163964

   Purpose

   Expression of TPMV F glycoprotein in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   TPMV F glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lacking 32 amino acids at C-terminus

   Promoter

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_HDRT_TRAC_CD19-CAR

   Plasmid

   #183473

   Purpose

   Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

   Inserts

   TRAC (TRAC Human)
   CD19-specific CAR transgene insert
   TRAC (TRAC Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAT203

    Plasmid

    #134880

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSM.wrmScarlet1-10::SL2::GFP1-10

    Plasmid

    #234318

    Purpose

    For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

    Insert

    wrmScarlet1-10::SL2::GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGal4BD-CRY2PHR

    Plasmid

    #28244

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    cry2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCG-TPMV Hd32

    Plasmid

    #163963

    Purpose

    Expresses TPMV H glycoprotein in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    TPMV H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 2 to 33 at N terminus deleted

    Promoter

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJC6-NADK2-3xFLAG

    Plasmid

    #233895

    Purpose

    lentiviral overexpression vector for NADK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    NADK2 (NADK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. SeAvs3

    Plasmid

    #188791

    Purpose

    Avs3 from Salmonella enterica NCTC13175

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    SeAvs3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET41sIII_GST-ALC1

    Plasmid

    #173932

    Purpose

    Bacterial expression of ALC1

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1 (CHD1L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCer-C3-Plk1-T210D

    Plasmid

    #68133

    Purpose

    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells

    Depositor

    Catherine Lindon

    Insert

    Polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    T210D constitutively active version

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pBS mFgfr1 (CT#92)

    Plasmid

    #14005

    Depositor

    Cliff Tabin

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pRcCMV myc-Plk1 K82R (Nigg LA184)

    Plasmid

    #41157

    Depositor

    Erich Nigg

    Article

    Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K82R

    Promoter

    CMV

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-STAT3mts

    Plasmid

    #195577

    Purpose

    Expresses STAT3 with mitochondrial targeting sequence

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Tags

    Cox8 presequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6.2-Nanoluc-ccdB

    Plasmid

    #87078

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTALYM16-Telo1

    Plasmid

    #47882

    Purpose

    TALE-mRuby2 against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PCS4 3XFLAG-PPARgamma2

    Plasmid

    #78770

    Purpose

    To overexpress PPARgamma2 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2 (Ppargc1a Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

31. p354_DG

    Plasmid

    #234697

    Purpose

    Golden gate domesticated pET28b for scarless cloning with His10-TEV

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138132

    Purpose

    Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEGFP-N1-LATS2

    Plasmid

    #166474

    Purpose

    Expresses fusion of mEGFP and LATS2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, LATS2 (LATS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAVS1P-iCLHN

    Plasmid

    #66579

    Purpose

    AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Nanoluc-HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

    Plasmid

    #176278

    Purpose

    Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p21 promoter A

    Plasmid

    #16462

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLEX-EF1a ANXA11-mEmerald D40G

    Plasmid

    #164211

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    D40G

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Hs.KRAS4b Q61H

    Plasmid

    #83140

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61H

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge