166,169 results

1. EC64420

   Plasmid

   #209517

   Purpose

   Cloning in complete guide expression cassettes, with Cas9

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-EGFR-L858R-tmpknot-epegRNA

   Plasmid

   #214092

   Purpose

   Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R epegRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYTK095

   Plasmid

   #65202

   Purpose

   Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA63_Helper

   Plasmid

   #190432

   Purpose

   M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   M13 genes: I-XI
   

   Use

   Synthetic Biology

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-rtTA

   Plasmid

   #175274

   Purpose

   AAV vector mediating neuron-specific expression of rtTA

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Reverse tetracycline-controlled transactivator (rtTA)
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry C1 Omp25

   Plasmid

   #157758

   Purpose

   Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   SYNJ2bp (SYNJ2BP Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-KRAB

   Plasmid

   #110820

   Purpose

   Conventional dCas9 repressor-dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHDM-VSV-G

   Plasmid

   #164440

   Purpose

   Encodes VSV-G protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   VSV-G
   

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

    Plasmid

    #138460

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-KRAB-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCVneo-HA-ER-Hoxb8

    Plasmid

    #222291

    Purpose

    The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    Mark Kamps, David Sykes

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon-optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Tags

    His-6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Promoter

    Simian CMV IE94

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid

    #105530

    Purpose

    AAV expression of EGFP from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPR-StAR4GN (Lenti)

    Plasmid

    #222693

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hygro-EF1A-EGFP-PARP1

    Plasmid

    #176146

    Purpose

    EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBMN(CMV-copGFP-Luc2-Puro)

    Plasmid

    #80389

    Purpose

    To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Luc2-F2A-Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYTK067

    Plasmid

    #65174

    Purpose

    Encodes ConR1 as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConR1
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti-CMV-NFE2L2-Puro

    Plasmid

    #181919

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGAL4-DBD-MCS

    Plasmid

    #145245

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSIQmate

    Plasmid

    #216240

    Purpose

    All-in One IQ-Cumate transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMRXIP TMEM41B-3xFLAG

    Plasmid

    #117415

    Purpose

    Expresses TMEM41B tagged with 3xFLAG in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. EF1a_FOXA1_P2A_Hygro_Barcode

    Plasmid

    #120438

    Purpose

    Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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31. pARC8-LambdaRedBeta-EcSSB

    Plasmid

    #162572

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. MSCV-C 16E7

    Plasmid

    #37880

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 16 E7 (E7 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pEGFP-NR2B

    Plasmid

    #17925

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl D-aspartate receptor 2B (Grin2b Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189925

    Purpose

    AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Use

    AAV

    Mutation

    SauriCas9 D15A

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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35. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mEmerald-CLIP170-C-18

    Plasmid

    #54043

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

37. pDEST51-LRRK2-R1441C

    Plasmid

    #25081

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Arginine 1441 mutated to Cysteine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21a-cys-His6-OtUBD

    Plasmid

    #190091

    Purpose

    Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge