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We narrowed to 14,406 results for: EGF

Showing: 5701 - 5750 of 14406 results

  1. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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  8. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid
    #223503
    Purpose
    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag and EGFP
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223503-plasmid-map-sequence-id-443301

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  11. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49235
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49235-plasmid-map-sequence-id-376544

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  13. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid
    #223136
    Purpose
    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized PEmax-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    M-MLV mutations from PE2 (Anzalone et al. Nature …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223136-plasmid-map-sequence-id-447644

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  14. pAAV-hSyn-Flex-Axon-EGFP

    Plasmid
    #135429
    Purpose
    Can be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Has Service
    AAV1
    Insert
    axon-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GAP43 palmitoylation domain
    Expression
    Mammalian
    Promoter
    hSyn1
    Available Since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135429-plasmid-map-sequence-id-271167

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  27. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Keith Joung
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    140083-plasmid-map-sequence-id-292705

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  32. pHR SFFV GLP1R(S301A)-EGFP

    Plasmid
    #158123
    Purpose
    Lentiviral plasmid for expression of GLP1R(S301)-EGFP fusion with SFFV promoter.
    Depositor
    Neal Devaraj
    Insert
    GLP1R (GLP1R )
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Serine 301 changed to alanine to enhance membrane…
    Promoter
    SFFV
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    158123-plasmid-map-sequence-id-309384

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  33. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid
    #191154
    Purpose
    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    EGFP-t70587
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV-TO
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191154-plasmid-map-sequence-id-383934

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  38. pLV-EGFP-Human-UTROPHIN_1-261

    Plasmid
    #222393
    Purpose
    Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and EGFP (UTRN Homo Sapiens, Human)
    Use
    Lentiviral
    Tags
    EGFP and Human UTROPHIN 1-261
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26737-G…
    Promoter
    CMV promoter and enhancer
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222393-plasmid-map-sequence-id-446518

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  42. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5701 - 5750 of 14406 results