We narrowed to 29,757 results for: REP

1. pEGFP_mEGFP-GluA1c

   Plasmid

   #114136

   Purpose

   Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mEGFP tagged GluA1 C-terminus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HPF1-c004

   Plasmid

   #216874

   Purpose

   Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   Full length HPF1 (HPF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_VNp6-DARPinOFF7

   Plasmid

   #182398

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-DARPinOFF7
   

   Use

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CT3G-CERP2-MG-IRES2-mCherry

   Plasmid

   #175507

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. APLF-c001

   Plasmid

   #173077

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   APLF (APLF Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K360-V448, codon optimized

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV TRE3G BCL2L11 Neo

   Plasmid

   #140747

   Purpose

   Inducibly expresses BIM in mammalian cells

   Depositor

   Scott Dixon

   Article

   Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

   Insert

   BCL2L11 (BCL2L11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT-GEM(2)

   Plasmid

   #62894

   Purpose

   Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-nbt-Electra2-P2A-mScarlet

   Plasmid

   #196114

   Purpose

   zebrafish expression of nbt-Electra2-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-Electra2-P2A-mScarlet
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCytERM-Electra2

    Plasmid

    #179477

    Purpose

    ER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-GCaMP3bright

    Plasmid

    #74423

    Purpose

    Express fluorescent calcium indicator GCaMP3bright in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3bright
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D392A

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBD_R44Q-IFP2.0C

    Plasmid

    #215749

    Purpose

    Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 2xHP1bCD-IFP2.0C

    Plasmid

    #215750

    Purpose

    Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH2B-Electra1

    Plasmid

    #179478

    Purpose

    Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    H2B-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-Lyn-SpiCee

    Plasmid

    #140837

    Purpose

    mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    Lyn-SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC Ad VAI

    Plasmid

    #175518

    Purpose

    For mammalian expression of adenovirus VAI RNA

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    Ad VAI RNA
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    lac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GOMO-GFP

    Plasmid

    #60975

    Purpose

    Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

    Depositor

    Olivier Silvie

    Article

    Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

    Inserts

    GFP
    mCherry
    hDHFR-yFCU
    

    Use

    Plasmodium gene targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT-GEM(1)

    Plasmid

    #62893

    Purpose

    Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-UGCG-KO

    Plasmid

    #80010

    Purpose

    gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    UGCG (UGCG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAD-SPS6-Pman

    Plasmid

    #133906

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-GCaMP3fast

    Plasmid

    #74422

    Purpose

    Express fluorescent calcium indicator GCaMP3fast in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3fast
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W4Y D356A

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMK284 (Stag-3FLAG-Hygro)

    Plasmid

    #72800

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Hygro
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCSIIbsr-4082NES(ForAMPK)

    Plasmid

    #170310

    Purpose

    The lentiviral vector for AMPK FRET-biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    4082NES (FRET biosensor for AMPK activity)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tensin-2 WT

    Plasmid

    #201797

    Purpose

    Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Use

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pPaxillin-Electra2

    Plasmid

    #184926

    Purpose

    Expresses paxillin fused to blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    paxillin-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pETM6-G6-vioABECD

    Plasmid

    #66536

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ARH1-c003

    Plasmid

    #173093

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRH (ADPRH Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-L357, codon optimized

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSFDuet-1_VNp-Uricase

    Plasmid

    #182401

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-uricase
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-GFAP(long)-FLEX-GCaMP7f

    Plasmid

    #189612

    Purpose

    Cre-dependent GCaMP7f under the GFAP promoter

    Depositor

    Martin Riccomagno

    Article

    Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

    Insert

    GCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    GCaMP7f

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMK283 (Stag-3FLAG-Neo)

    Plasmid

    #72799

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Neo
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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