We narrowed to 9,346 results for: UTY
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Plasmid#171811PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmScarlet-F-mgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]
Plasmid#171810PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmScarlet-F-hgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-Ova-BlastR [M1G]
Plasmid#171808PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1GDepositorInsertmNeonGreen-F-Ova-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-gB-BlastR [M1G]
Plasmid#171807PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1GDepositorInsertmNeonGreen-F-gB-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-BlastR [M1G]
Plasmid#171806PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]DepositorInsertmNeonGreen-F-hgp100-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-Ova-PuroR [M1G]
Plasmid#171804PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-Ova-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(151-154AAAA)
Plasmid#166131PurposeExpression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.DepositorInsertT1head1-405(151-154AAAA) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationaa1-405 only. Contains 4 amino acid substitutions…PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Cstem(7A)-Tac(5A)
Plasmid#162499PurposeExpresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).DepositorInsertUseTagsGFPExpressionMammalianMutationThe five theronine and two serine residues are mu…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
Plasmid#156413Purposekdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sitesDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseZebrafish transgenesisTagsEYFPExpressionMutationPromoterkdrlAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)
Plasmid#156404PurposeGateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseGateway cloningTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2/deltaILTM4
Plasmid#119735PurposeGABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-termDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
DRH015_ AAV-hSyn-WT-THR
Plasmid#225088PurposeExpression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.DepositorInsertWT-THRB (THRB Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRH016_ AAV-hSyn-DN-THR
Plasmid#225089PurposeExpression of dominant-negative thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.DepositorInsertDN-THRB (THRB Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 nonmutant
Plasmid#183813PurposeExpress CD74-ROS1 fusion (C6;R34) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 G2032R
Plasmid#183814PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cellsDepositorUseRetroviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationG2032RPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2086F
Plasmid#183816PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorInsertAIMTOR A757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
hNTa1-qgRNA-pYJA5
Plasmid#217779PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6, mouse U6, human H1, human 7SKAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNTo1-qgRNA-pYJA5
Plasmid#217781PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6, mouse U6, human H1, human 7SKAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNTo2-qgRNA-pYJA5
Plasmid#217782PurposeNon-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6, mouse U6, human H1, human 7SKAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2000V
Plasmid#183815PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2000VPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
hNTa2-qgRNA-pYJA5
Plasmid#217780PurposeNon-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6, mouse U6, human H1, human 7SKAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
DRH031_ AAV-SIO-nEF-WT-THR
Plasmid#225090PurposeCre-dependent expression of wild-type thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.DepositorInsertDN-THRB (THRB Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRH032_ AAV-SIO-nEF-DN-THR
Plasmid#225091PurposeCre-dependent expression of dominant-negative thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.DepositorInsertWT-THRB (THRB Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a
Plasmid#183829PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 1
Plasmid#183828PurposeExpress EML4-ALK fusion variant 1 (E13;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-13 and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 G2032R/L2086F
Plasmid#183820PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationG2032R/L2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.2 Spike
Plasmid#185604PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2 SpikeDepositorInsertSARS-CoV-2 BA.2 Spike (S )
UseTagsExpressionMammalianMutationT19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJuly 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-CD74-ROS1 S1986F/G2032R/L2086F
Plasmid#183822PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/G2032R/L2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-CD74-ROS1 S1986F/G2032R
Plasmid#183821PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/G2032RPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 S1986F/L2000V
Plasmid#183818PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/L2000VPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 S1986F/L2086F
Plasmid#183819PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/L2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.1 Spike
Plasmid#185452PurposeExpresses codon-optimized full length SARS-CoV-2 BA.1 SpikeDepositorInsertSARS-CoV-2 Spike BA.1 (S )
UseTagsExpressionMammalianMutationContains the following mutations: A67V, Δ69-70, T…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-EML4-ALK variant 3a G1202R
Plasmid#183830PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationG1202RPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a L1196M/G1202R
Plasmid#183831PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationL1196M/G1202RPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 F276C 3xFlag
Plasmid#110064PurposeFGFR2 F276C expression in mammalian cells (JCOPO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Phenylalanine 276 to Cysteine (F276C)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K41E 3xFlag
Plasmid#110105Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 41 to Glutamic acid (K41E)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.MCS.IRES.GFP
Plasmid#169280PurposeConstitutive overexpression of cDNA with GFP as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.MCS.IRES.dTomato
Plasmid#169279PurposeConstitutive overexpression of cDNA with dTomato as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SIN40C.SFFV.dTomato.miR30n
Plasmid#169277PurposeConstitutive overexpression of miRNA or shRNA with dTomato as reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.GFP.miR30n
Plasmid#169278PurposeConstitutive overexpression of miRNA or shRNA with GFP as reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -