19,190 results

1. pSicoR-eIF1a-GFP-RPL10a

   Plasmid

   #159890

   Purpose

   Expresses GFP-RPL10a for ribosomal immunoprecipitation

   Depositor

   James Ellis

   Article

   Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

   Insert

   RPL10a (RPL10A Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   eIF1a

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPS22 (pET23a-Cas10/Csm/csm2H6N)

   Plasmid

   #70038

   Purpose

   Bacterial expression of the S. epidermidis Cas10·Csm complex

   Depositor

   Luciano Marraffini

   Article

   Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

   Insert

   Cas10/Csm complex
   

   Use

   Tags

   6xHIS on N terminus of Csm2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSin Capsid

   Plasmid

   #127693

   Purpose

   Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Capsid
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pKEN GFP mut2

   Plasmid

   #20409

   Depositor

   Raphael Valdivia, Stanley Falkow

   Article

   Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S65A V68L S72A

   Promoter

   Available since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPet-His

   Plasmid

   #14031

   Depositor

   Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

   Insert

   YPet
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-NPC1(WT)-FLAG

   Plasmid

   #164972

   Purpose

   Lentiviral vector for NPC1 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-HA

   Plasmid

   #101767

   Purpose

   Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

    Plasmid

    #176541

    Purpose

    human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB omegaRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. scFLARE1

    Plasmid

    #158738

    Purpose

    Mammalian expression of scFLARE1 component

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE1
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hPDGFRA-GFP

    Plasmid

    #22919

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSKBAD2

    Plasmid

    #15335

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-OMM-GCaMP6f

    Plasmid

    #127874

    Purpose

    Fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Outer membrane localized GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVITRO-HPV18 L1L2

    Plasmid

    #52592

    Purpose

    Expresses HPV18 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV18 L1
    HPV18 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. CapChR2_pmScarlet-N1

    Plasmid

    #188032

    Purpose

    Expresses CapChR2-mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINE GFP nanobody

    Plasmid

    #49172

    Purpose

    Bacterial expression of GFP nano body

    Depositor

    Brett Collins

    Article

    Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

    Insert

    GFP nanobody
    

    Use

    Tags

    HHHHHH

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHes7-Achilles-Hes7

    Plasmid

    #153528

    Purpose

    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

    Depositor

    Ryoichiro Kageyama

    Article

    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

    Insert

    Hes7 (Hes7 Mouse)
    

    Use

    Tags

    Achilles

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hes7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. CMV-Sp1

    Plasmid

    #12097

    Depositor

    Robert Tjian

    Insert

    Sp1 (SP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-87 deleted

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS-Int

    Plasmid

    #50000

    Purpose

    Expresses the integrase on a suicide plasmid

    Depositor

    Peter Sander, Brian Robertson

    Article

    Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

    Insert

    Integrase from pMV361
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    own

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pUGI-NLS

    Plasmid

    #101091

    Purpose

    Expresses UGI in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBT120a17

    Plasmid

    #138510

    Purpose

    P1

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    gIII-xLuxAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA6-N-3XFLAG-Ctnnb1

    Plasmid

    #123586

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDH-puro-cMyc

    Plasmid

    #46970

    Purpose

    lentiviral expression of c-Myc

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR1A-GFP-N2 (FR1)

    Plasmid

    #19364

    Purpose

    Gateway entry vector for C-term eGFP fusion, frame N2

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-HIF1alpha P402A/P564A-pcDNA3

    Plasmid

    #18955

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCh-Climp63

    Plasmid

    #136293

    Purpose

    expression of Climp63

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Cytoskeletal-linking protein 63 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge