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Showing: 4681 - 4720 of 17176 results

  11. pMIA10.7

    Plasmid
    #214451
    Purpose
    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Inserts
    Tet-On 3G
    PuroR
    TRE3G CopGFP
    Use
    Bxbi donor
    Tags
    PuroR, T2A, and Tet-On 3G
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214451-plasmid-map-sequence-id-431689

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  16. pJR100

    Plasmid
    #187240
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    187240-plasmid-map-sequence-id-374768

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  18. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Article
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    84041-plasmid-map-sequence-id-164306

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  19. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid
    #183790
    Purpose
    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.
    Depositor
    Jay Shendure
    Article
    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-
    Inserts
    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    G>A in the gRNA scaffold- please see depositor…
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183790-plasmid-map-sequence-id-374366

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  25. lenti-EGFR-L858R-T790M-dual-epegRNA

    Plasmid
    #214100
    Purpose
    Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.
    Depositor
    Ting Zhou
    Article
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    EGFR L858R epegRNA/EGFR T790M epegRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214100-plasmid-map-sequence-id-429653

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Showing: 4681 - 4720 of 17176 results