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We narrowed to 11,193 results for: ENA

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Showing: 5851 - 5900 of 11193 results

  1. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid
    #140163
    Purpose
    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker
    Depositor
    Benjamin Glick
    Article
    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.
    Insert
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140163-plasmid-map-sequence-id-275937

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  9. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid
    #233348
    Purpose
    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted (Cyb5a 95-134) and N538E
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233348-plasmid-map-sequence-id-466824

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  10. LSB-hsa-let-7d-5p

    Plasmid
    #103151
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7d-5p target (MIRLET7D Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103151-plasmid-map-sequence-id-213230

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  11. LSB-hsa-miR-21-5p

    Plasmid
    #103345
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-21-5p target (MIR21 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103345-plasmid-map-sequence-id-195739

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  14. LSB-hsa-let-7b-5p

    Plasmid
    #103148
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7b-5p target (MIRLET7B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103148-plasmid-map-sequence-id-213110

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  22. LSB-hsa-miR-548e-5p

    Plasmid
    #103634
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-548e-5p target (MIR548E Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103634-plasmid-map-sequence-id-196316

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  25. pcDNA3.1.H1-NP

    Plasmid
    #134367
    Purpose
    Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    H1-NP (HRH1 Human)
    Tags
    Flag and natural peptide of nanoluciferase
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134367-plasmid-map-sequence-id-306386

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  33. LSB-hsa-miR-132-3p

    Plasmid
    #103219
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-132-3p target (MIR132 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103219-plasmid-map-sequence-id-195487

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  37. LSB-hsa-miR-27a-3p

    Plasmid
    #103381
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-27a-3p target (MIR27A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103381-plasmid-map-sequence-id-195811

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  38. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. LSB-hsa-miR-133b

    Plasmid
    #103223
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-133b target (MIR133B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103223-plasmid-map-sequence-id-195495

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  46. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid
    #208967
    Purpose
    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208967-plasmid-map-sequence-id-415814

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  49. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  50. LSB-hsa-miR-195-3p

    Plasmid
    #103311
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-195-3p target (MIR195 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103311-plasmid-map-sequence-id-195671

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Showing: 5851 - 5900 of 11193 results