We narrowed to 17,356 results for: URE

1. pTH1-SpSrtA

   Plasmid

   #64987

   Purpose

   Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   S. pyogenes sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-81

   Promoter

   T7 promoter

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIS.MTRIA-5HT

   Plasmid

   #184597

   Purpose

   For expression of a fluorescent sensor for serotonin in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-5HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAK002

   Plasmid

   #128215

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 2

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. A0A075HNX4

   Plasmid

   #177695

   Purpose

   expresses a fatty alcohol oxidase in E. coli

   Depositor

   Martin Engqvist

   Article

   Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

   Insert

   Long chain alcohol oxidase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFP-Rab33bb in pCSDEST2

   Plasmid

   #140882

   Purpose

   Expression of zebrafish GFP-tagged Rab33bb in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab33bb
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUE1-TSP-UL32

   Plasmid

   #162658

   Purpose

   Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   UL32
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpCas9n(BB)-2A-puro-a5 guide B

   Plasmid

   #194011

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide B)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpCas9n(BB)-2A-puro-aV guide A

   Plasmid

   #194008

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9n(BB)-2A-puro-aV guide B

   Plasmid

   #194009

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide B)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9n(BB)-2A-puro-a5 guide A

    Plasmid

    #194010

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

    Plasmid

    #84254

    Purpose

    Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sa sgCXCR4-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCellFree_G03 WWP1

    Plasmid

    #67125

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TwinStrep-SUMO-Pdp1

    Plasmid

    #198320

    Purpose

    For affinity purification of Pdp1 payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pdp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs-ERas

    Plasmid

    #13355

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    ES cell expressed Ras (Eras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pFH2.21_mCherry-MS2x6

    Plasmid

    #205536

    Purpose

    Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-mScarlet-STOP

    Plasmid

    #140876

    Purpose

    Multisite Gateway middle entry vector for expression of untagged mScarlet.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mScarlet
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4092694

    Plasmid

    #153907

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. CSII-TK-MCS

    Plasmid

    #203597

    Purpose

    Empty lentiviral vector to express protein in mammalian cells cells.

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TK

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-mWnt2

    Plasmid

    #42274

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Wnt2 (Wnt2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2013

    Availability

    Academic Institutions and Nonprofits only

20. MDH1-PGK-GFP microRNA-10b

    Plasmid

    #16070

    Depositor

    Bob Weinberg

    Article

    Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

    Insert

    microRNA-10b (MIR10B Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pBS MCK CRE

    Plasmid

    #12529

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Transgenic construct

    Tags

    Expression

    Mutation

    Cre is driven by the Muscle Creatine Kinase promo…

    Promoter

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCellFree_G04

    Plasmid

    #67138

    Purpose

    Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and HIS

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAK008

    Plasmid

    #128225

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 2

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAK009

    Plasmid

    #128226

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCIS.MTRIA-NPY

    Plasmid

    #184613

    Purpose

    For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NPY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCellFree_G02

    Plasmid

    #67136

    Purpose

    Gateway destination vector for cell-free expression with C-term 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHCMV_EBLV-1_G

    Plasmid

    #207270

    Purpose

    Mammalian expression of European bat lyssavirus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    EBLV-1_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLBH546_Tet-Cas14b1Locus

    Plasmid

    #112503

    Purpose

    Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pUC19-URA3-3'SEC13-GFP

    Plasmid

    #25445

    Depositor

    Benjamin Glick

    Article

    Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

    Insert

    Sec13-Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMaCTag-P31

    Plasmid

    #120042

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    V5
    

    Use

    Pcr template

    Tags

    V5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b/GgVcl 1-1066 (full length)

    Plasmid

    #46171

    Depositor

    Susan Craig

    Article

    Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

    Insert

    Vcl (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMaCTag-12

    Plasmid

    #119991

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCIS.MTRIA-SST

    Plasmid

    #184619

    Purpose

    For expression of a fluorescent sensor for somatostatin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-SST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mCherry_LD 0_TEV

    Plasmid

    #58868

    Purpose

    MESA protease chain with mCherry ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with mCherry ectodomain and TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FCK-QuasAr2-mOrange2 eFRET

    Plasmid

    #59173

    Purpose

    Fluorescent membrane voltage sensor

    Depositor

    Adam Cohen

    Article

    Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

    Insert

    QuasAr2-mOrange2 eFRET
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

    Plasmid

    #216382

    Purpose

    Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Tetraspanin-12 (TSPAN12 Human)
    

    Use

    Baculovirus

    Tags

    6xHis-3C-splitGFP11

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLBH547_Tet-Cas14b2Locus

    Plasmid

    #112504

    Purpose

    Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV.PARP1#5

    Plasmid

    #14548

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pCellFree_G03 TFAP2B

    Plasmid

    #67092

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Sc eRF1 (WT)

    Plasmid

    #175413

    Purpose

    Encodes His-MBP-TEV-WT eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

    Plasmid

    #116963

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pmCellFree_KA2

    Plasmid

    #145370

    Purpose

    Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Type

    Empty backbone

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pME-Lifeact-mEos2-NS

    Plasmid

    #115549

    Purpose

    Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Lifeact-mEos2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. py100-sgRNA_hVEGFA

    Plasmid

    #198282

    Purpose

    Expression of SpCas9 guide RNA specific for human VEGFA locus

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    py100-sgRNA_hVEGFA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMS17: pTwinStrep-SUMO-AvTnsD

    Plasmid

    #168150

    Purpose

    AvTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsD
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLV.PARP1#4

    Plasmid

    #14547

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

49. psiCHECK3-TMED3

    Plasmid

    #136014

    Purpose

    TMED3 3'UTR (NM_007364.3) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    TMED3 3'UTR (TMED3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. TwinStrep-SUMO-Pnf

    Plasmid

    #198321

    Purpose

    For affinity purification of Pnf payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pnf
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge