4,272 results

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phage UbiC G3BP1-GFP-GFP

Plasmid

#119950

Purpose

Lentiviral vector expressing G3BP1-GFP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6c

Plasmid

#207853

Purpose

Mammalian expression of PE6c prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6c

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry

Plasmid

#114472

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

hSyn

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-Luc-ZsGreen

Plasmid

#39196

Depositor

Bryan Welm

Inserts

Firefly Luciferase Luc2P
ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha and EF1-alpha

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Article

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Blast empty (w263-1)

Plasmid

#17486

Purpose

3rd gen lentiviral empty control vector, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+mNeonGreen-C Cloning Vector

Plasmid

#128144

Purpose

To express a protein of interest fused to the C-terminus of mNeonGreen

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

mNeonGreen fusion

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

Plasmid

#71396

Purpose

2Gi2R assay

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Inserts

2xGFP:NES
2xRFP:NLS

Use

Lentiviral

Tags

IRES

Expression

Mammalian

Promoter

EF1alpha and IRES

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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Human SERCA2a (pcDNA3.1+)

Plasmid

#75187

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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