165,947 results

1. Orai1-GCaMP6f

   Plasmid

   #73564

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   Orai1-GCaMP6f (ORAI1 Human, Synthetic)
   

   Tags

   GCaMP6f

   Expression

   Mammalian

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TMEM106B-EGFP

   Plasmid

   #210366

   Purpose

   Expresses TMEM106B fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   TMEM106B (TMEM106B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBPnlsLexA::p65Uw

   Plasmid

   #26230

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::p65 (RELA Human)
   

   Expression

   Insect

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

   Plasmid

   #153005

   Purpose

   Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (C) (G79)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFLAG_WT MDA5

   Plasmid

   #216798

   Purpose

   Expresses wild type (wt) melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Expression

   Mammalian

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-FGF2-G3

   Plasmid

   #135521

   Purpose

   Engineered form of fibroblast growth factor 2 with improved thermostability

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   6x His tag and Agg Thrombin Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cbh_v5 AAV-ABE C-terminal

   Plasmid

   #137178

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-UPEmS-tevo

   Plasmid

   #228894

   Purpose

   Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Caspase1-flag

   Plasmid

   #21142

   Depositor

   Junying Yuan

   Article

   Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

   Insert

   caspase-1 (Casp1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTARA

    Plasmid

    #31491

    Depositor

    Kathleen Matthews

    Article

    Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

    Insert

    RNA polymerase
    

    Expression

    Bacterial

    Mutation

    N823D

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ8001 - pCMV-R8.91 (D64V)

    Plasmid

    #202687

    Purpose

    R8.91 plasmid with mutated integrase (D64V)

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    gag pol
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D64V

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. nuclear cpVenus control

    Plasmid

    #186790

    Purpose

    Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cbh_v5 AAV-ABE N-terminal

    Plasmid

    #137177

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-ABE N-terminal
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP Centrin2 (Nigg UK185)

    Plasmid

    #41147

    Depositor

    Erich Nigg

    Article

    Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

    Insert

    Centrin-2 (CETN2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0731

    Plasmid

    #142201

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PROX1 (PROX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH2B-Electra2

    Plasmid

    #179479

    Purpose

    Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    H2B-Electra2
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Bxb1-LP-v2-TC

    Plasmid

    #194326

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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