We narrowed to 4,490 results for: Gca

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pAAV-Ef1a-Con/Foff 2.0-GCaMP6F

Plasmid

#137123

Purpose

Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

Plasmid

#137120

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-H2B-jGCaMP8m-WPRE

Plasmid

#176748

Purpose

AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

H2B-jGCaMP8m

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F2bbs kan GCANNNAGGk

Plasmid

#18752

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pJYS2_crtYf

Plasmid

#85544

Purpose

Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-GEX-GECO1

Plasmid

#32443

Purpose

Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEX-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-8x-gRNA-eGFP

Plasmid

#60718

Purpose

eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Anillin

Plasmid

#68027

Purpose

Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Article

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Insert

Anillin (ANLN Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations at 798 5'-TGCC TCTTTGAATAAA…

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_GCAMP7s

Plasmid

#182940

Purpose

alpha7 nACHR expression in mammalian cell lines with calcium reporter

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCgCas9_recT

Plasmid

#153337

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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