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We narrowed to 9,406 results for: Coli

Showing: 5951 - 6000 of 9406 results

  1. pUC19-P70a-Top-T

    Plasmid
    #87312
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli system "Top" transcript
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available Since
    Jan. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    87312-plasmid-map-sequence-id-201363

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  4. pFLIP30

    Plasmid
    #65499
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-mCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65499-plasmid-map-sequence-id-111219

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  5. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid
    #73221
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL31-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL31
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73221-plasmid-map-sequence-id-135054

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  6. pTH09

    Plasmid
    #70064
    Purpose
    artificial transcription template "terminator insertion"
    Depositor
    Jeff Gelles
    Article
    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi
    Insert
    lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pFLIP32

    Plasmid
    #65500
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65500-plasmid-map-sequence-id-111220

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  8. pFLIP39

    Plasmid
    #65507
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65507-plasmid-map-sequence-id-111416

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  10. pCMK83

    Plasmid
    #226793
    Purpose
    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Insert
    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline resistance cassette promoter (consti…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    226793-plasmid-map-sequence-id-455428

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  15. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222017
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    deleted stop codon (TAA)
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222017-plasmid-map-sequence-id-457152

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  27. pJB75

    Plasmid
    #226320
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis-(Y to A MR1-6)-strep
    Use
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226320-plasmid-map-sequence-id-449984

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  28. pJB67

    Plasmid
    #226316
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with K to A CsoS2 MR1-6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K261A, K320A, K380A, K436A, K495A, K546A
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226316-plasmid-map-sequence-id-449928

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  29. pJB74

    Plasmid
    #226314
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1-5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A, Y359A, Y420A, Y475A, Y534A
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226314-plasmid-map-sequence-id-449926

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  30. pJB73

    Plasmid
    #226313
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1-3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A, Y359A, Y420A
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226313-plasmid-map-sequence-id-449925

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  31. pJB72

    Plasmid
    #226312
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226312-plasmid-map-sequence-id-449924

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  32. pJB45

    Plasmid
    #226304
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S CsoS2 MR1-2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S, C351S, C369S
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226304-plasmid-map-sequence-id-449916

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  33. pJB44

    Plasmid
    #226303
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226303-plasmid-map-sequence-id-449915

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  34. pJB43

    Plasmid
    #226302
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    WT CsoS2, frameshift site intact
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226302-plasmid-map-sequence-id-449914

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  39. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208628-plasmid-map-sequence-id-433639

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  40. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208625-plasmid-map-sequence-id-433638

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  41. pET-6xHis-GFP(+6)-L1

    Plasmid
    #205039
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-L1
    Use
    Tags
    GRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205039-plasmid-map-sequence-id-405652

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  42. pET-6xHis-GFP(+6)-L2

    Plasmid
    #205040
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-L2
    Use
    Tags
    GRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205040-plasmid-map-sequence-id-405653

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  43. pET-6xHis-GFP(+6)-L3

    Plasmid
    #205041
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-L3
    Use
    Tags
    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205041-plasmid-map-sequence-id-405654

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  44. pET-6xHis-GFP(+6)-H1

    Plasmid
    #205042
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-H1
    Use
    Tags
    KRRRKK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205042-plasmid-map-sequence-id-405655

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  45. pET-6xHis-GFP(+6)-H2

    Plasmid
    #205043
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-H2
    Use
    Tags
    KRRRKKKRRRKK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205043-plasmid-map-sequence-id-405656

    Enlarge

  46. pET-6xHis-GFP(+6)-H3

    Plasmid
    #205044
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-H3
    Use
    Tags
    KRRRKKKRRRKKKRRRKK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205044-plasmid-map-sequence-id-405657

    Enlarge

  49. pET-6xHis-GFP(0)-L2

    Plasmid
    #205034
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(0)-L2
    Use
    Tags
    GRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, I138R, D207R, N222K, L246R
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205034-plasmid-map-sequence-id-405644

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  50. pET-6xHis-GFP(0)-L3

    Plasmid
    #205035
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(0)-L3
    Use
    Tags
    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, I138R, D207R, N222K, L246R
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205035-plasmid-map-sequence-id-405646

    Enlarge

Showing: 5951 - 6000 of 9406 results