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We narrowed to 5,868 results for: nls

Showing: 4801 - 4840 of 5868 results

  1. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

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  2. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  3. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  4. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  5. pGfa2(ABDx3)-nLac

    Plasmid
    #53133
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of 3 copies of the gf…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53133-plasmid-map-sequence-id-189228

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  6. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  10. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid
    #177347
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177347-plasmid-map-sequence-id-353618

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  11. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135014-plasmid-map-sequence-id-267599

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  13. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135013-plasmid-map-sequence-id-267601

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  17. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  18. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid
    #136652
    Purpose
    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136652-plasmid-map-sequence-id-273328

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  23. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid
    #199263
    Purpose
    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199263-plasmid-map-sequence-id-389813

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  26. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  27. pEMS1401

    Plasmid
    #29198
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29198-plasmid-map-sequence-id-13613

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  31. pGfa(B3)-nLac

    Plasmid
    #53132
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of the gfa2 B segment…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53132-plasmid-map-sequence-id-82721

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  35. pEMS1538

    Plasmid
    #29261
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    Oct. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29261-plasmid-map-sequence-id-13676

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  38. AAV-hSyn-scFVDnmt3AL_bGHpA

    Plasmid
    #177348
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from Synapisin promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    human Synapsine
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177348-plasmid-map-sequence-id-353661

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  39. AAV-TRE-scFVDnmt3AL_bGHpA

    Plasmid
    #177354
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177354-plasmid-map-sequence-id-353614

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Showing: 4801 - 4840 of 5868 results