159,557 results

1. pCMU-PERr

   Plasmid

   #61202

   Purpose

   Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   mCherry-SKL
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-AncBE4max

   Plasmid

   #174164

   Purpose

   This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

   Depositor

   Wensheng Wei

   Article

   Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

   Insert

   AncBE4max
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Puro cTnT FUCCI

   Plasmid

   #136935

   Purpose

   donor plasmid for cardiac-specific FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Troponin T promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cTnT

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EFS-MCP-HALOnls

   Plasmid

   #121937

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   MCP-HALOnls
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-SNAP-PI Probe

   Plasmid

   #211507

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BcPI-PLC-ANH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

   Plasmid

   #216694

   Purpose

   Synchronize trafficking of GPI-AP from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   SBP-mApple-FIREtag-GPI
   

   Use

   Tags

   FIREtag, IL2 signal sequence, Streptavidin Bindin…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-Sox2

   Plasmid

   #13367

   Purpose

   Retroviral expression of mouse Sox2

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   SRY-box containing gene 2 (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-inducible Luciferase reporter

   Plasmid

   #64127

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetOx13-CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCARZ-CBh-GFP-SV40PA-GF3-R4A22

   Plasmid

   #203767

   Purpose

   For production of template RNA for use in PRINT, GFP under CBh promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for GFP
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lyso-GA

    Plasmid

    #209874

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPEgRNA

    Plasmid

    #172716

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mNeonGreen-mTurquoise2

    Plasmid

    #98886

    Purpose

    Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Use

    Tags

    GFP11x7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nuclear cpGFP

    Plasmid

    #215707

    Purpose

    Expresses cpGFP in the nucleus

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNAPf-Cox8A Control Plasmid

    Plasmid

    #101129

    Purpose

    Expression of SNAP-Tagged Cox8A in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Cox8A (COX8A Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3.1 TaGu N-1F

    Plasmid

    #203762

    Purpose

    For expression of N-flag tagged TaGu R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACgp67B-Her2

    Plasmid

    #10794

    Depositor

    Judy Lieberman

    Article

    Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

    Insert

    ErbB2 ScFv (ERBB2 Human)
    

    Use

    Tags

    6His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

    Plasmid

    #224006

    Purpose

    AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234451

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHMM

    Plasmid

    #67717

    Purpose

    Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Hexahistidine/Maltose binding protein/MS2 coat protein fusion
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. hGip-R

    Plasmid

    #14942

    Depositor

    Bernard Thorens

    Article

    Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

    Insert

    Gip-R (GIPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Short form of the receptor

    Promoter

    Available since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3-RAB-ICUE

    Plasmid

    #118480

    Purpose

    ddRFP-based single-color biosensor for monitoring cAMP dynamics.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-ICUE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Use

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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26. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJR98

    Plasmid

    #187239

    Purpose

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

    Plasmid

    #125228

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge