167,150 results
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Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
gTEMP_pcDNA3
Plasmid#89583PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertgTEMP
ExpressionMammalianPromoterCMVAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF-miRFP670v1-hGem(1/110)
Plasmid#80006PurposehGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)DepositorAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD-mKate(KPS12)
Plasmid#133831PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPuppAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Tau-cYFP
Plasmid#92205PurposeRetroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)DepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pES002 MBP-GSK3β_S9A-HA-His
Plasmid#196184PurposeExpresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
TagsHA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShHELIX-KanR (CJT70)
Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-Nir1-LNS2
Plasmid#220084Purposehigh affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)DepositorInsertPITPNM3 (PITPNM3 Human)
TagsmCherry-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
All-in-one lenti-ABE8e-NG
Plasmid#200447PurposeLentivirus vector for base editingDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#194703PurposeInducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsAPOBEC1-HA-P2A-mRubyExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
ExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) NAPstar3b
Plasmid#232736PurposeExpresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.DepositorInsertNAPstar3b
ExpressionMammalianAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X TE Down (GQs) Renilla
Plasmid#70039PurposePlasmid for testing 5’UTR TE Down (G-quadruplex) folding elementsDepositorInsert4x TE Down (G-quadruplexes) in 5'UTR
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutation4 repeats of TE down motif (G-quadruplexes) in 5&…PromoterSV40Available SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X Mut Renilla
Plasmid#70040PurposeControl plasmid for testing 5'UTR folding elementsDepositorInsertRandom sequence matched for GC-content
UseLuciferaseTagsrenilla luciferaseExpressionMammalianPromoterSV40Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TCF7L2_WT
Plasmid#81769PurposeGateway Donor vector containing TCF7L2 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRPromoterJ23119Available SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-OSKM
Plasmid#20321PurposeLentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generationDepositorAvailable SinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE10
Plasmid#90344PurposeIRF3 - Viral response gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterIRF3Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40
Plasmid#100054PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activationDepositorHas ServiceAAV1, AAV2, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationH134R mutation in ChR2PromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-N1
Plasmid#54493PurposeLocalization: N1 Cloning Vector, Excitation: 558, Emission: 583DepositorTypeEmpty backboneTagsDsRed2ExpressionMammalianAvailable SinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W
Plasmid#67977PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markersDepositorInsertU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1
Plasmid#70759Purposebacterial expression of human XRCC1DepositorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72114-BBa_J72100
Plasmid#40781DepositorInsertlacZ
UseSynthetic Biology; PhagemidExpressionBacterialAvailable SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJA54 HLA-A*03:01
Plasmid#180451PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*03:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 puro Nodamura B2
Plasmid#17228DepositorInsertNodamura B2 (NoVsRNA1gp2 Nodamura Virus)
ExpressionMammalianMutation2 silent point mutationsAvailable SinceJune 22, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC13N-dCas9-BFP-KRAB
Plasmid#127968Purposeconstitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)DepositorInsertCLYBL-CAG-dCas9-NLS-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only