4,134 results

1. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF.hHES1.Ubc.GFP

   Plasmid

   #17624

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   HES1 (HES1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tubp-Gal80 in pCaSpeR4

   Plasmid

   #17748

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Tags

   SV40 polyA and Tubulin 1alpha promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCINeo-myc-Itch/2859

   Plasmid

   #11427

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Itch (Itch Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-ALN

   Plasmid

   #43918

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Gylcine-serine-glycine (GSG) linker followed by v…

   Promoter

   TRE promoter, Tet-ON

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDS-RED-C1-Beclin1

   Plasmid

   #24405

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLPo

   Plasmid

   #13792

   Purpose

   Expression of mouse codon-optimized FLP recombinase

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

   Insert

   FLPo
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPSmOrange-N1

   Plasmid

   #31898

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

   Insert

   PSmOrange
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-ERK2 WT

    Plasmid

    #8974

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-myc-Cdc42-Q61L

    Plasmid

    #12974

    Depositor

    Gary Bokoch

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Beclin1

    Plasmid

    #21150

    Depositor

    Junying Yuan

    Article

    Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

    Insert

    PcDNA3-Beclin1 (BECN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMK288 (mAID-Bsr)

    Plasmid

    #72826

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Bsr
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP(C3)-Vinculin

    Plasmid

    #30312

    Depositor

    Klaus Hahn

    Insert

    Vinculin (Vcl Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    F633S, V828E, L936P in mouse viniculin (NM_009502…

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 MEKK1 WT

    Plasmid

    #12181

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3xFLAG-MKL1

    Plasmid

    #11978

    Purpose

    For mammalian expression of human MKL1 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N579S T716A

    Promoter

    CMV

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVU/RED

    Plasmid

    #24178

    Depositor

    Lars Ittner

    Article

    Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    DsRED

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eTC GFP beta-actin full length

    Plasmid

    #27123

    Depositor

    Robert Singer

    Article

    Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

    Insert

    beta-actin (ACTB Human)
    

    Use

    Tags

    EGFP and TC tag

    Expression

    Mammalian

    Mutation

    E237A

    Promoter

    Available since

    Jan. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP C3-Lats1

    Plasmid

    #19053

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Large tumor suppressor kinase 1, N mutant (LATS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    **

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-AP-CFP-TM

    Plasmid

    #20861

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    AP-CFP-TM
    

    Use

    Tags

    CFP, HA, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. c-myc promoter (Del 4)

    Plasmid

    #16604

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 4) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-4 (PvuII->PvuII). The first PvuII site is …

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBABE-puro-mTwist

    Plasmid

    #1783

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZac2.1 gfaABC1D-lck-GCaMP6f

    Plasmid

    #52924

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Has Service

    AAV5

    Insert

    lck-GCaMP6f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pdCas9::BFP-humanized

    Plasmid

    #44247

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 fused to BFP
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS & tagBFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1 myc TSC1

    Plasmid

    #12133

    Depositor

    Cheryl Walker

    Article

    Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-EGFP-C1-HsRCK

    Plasmid

    #25033

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    Rck (DDX6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GolgiCFP

    Plasmid

    #14873

    Depositor

    Alexandra Newton

    Article

    Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

    Insert

    CFP
    

    Use

    Tags

    eNOS(1-35)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SNX9-pmCherryC1

    Plasmid

    #27678

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EdTP

    Plasmid

    #24311

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge