19,200 results

1. pHAGE-TO-dCas9-3XGFP

   Plasmid

   #64107

   Purpose

   Expression of Sp dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH0001

   Plasmid

   #85969

   Purpose

   UCOE-SFFV-dCas9-BFP-KRAB

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BFP-KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   SFFV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSKDuet01

   Plasmid

   #12172

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   NpuDnaE
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-TFR-20

   Plasmid

   #55144

   Purpose

   Localization: Transferrin Receptor, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TFR (TFRC Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Plasmid

   #163058

   Purpose

   roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   roGFP2-Orp1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPS22 (pET23a-Cas10/Csm/csm2H6N)

   Plasmid

   #70038

   Purpose

   Bacterial expression of the S. epidermidis Cas10·Csm complex

   Depositor

   Luciano Marraffini

   Article

   Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

   Insert

   Cas10/Csm complex
   

   Use

   Tags

   6xHIS on N terminus of Csm2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT120a17

   Plasmid

   #138510

   Purpose

   P1

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   gIII-xLuxAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-CD36-C-10

   Plasmid

   #55011

   Purpose

   Localization: Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD36 (CD36 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1/Ndi-1/myc-His

    Plasmid

    #127503

    Purpose

    Expresses myc-tagged yeast Ndi-1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    Ndi-1 (NDI1 Budding Yeast)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cx43-msfGFP

    Plasmid

    #69007

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Gja1 (Gja1 Rat)
    

    Use

    Tags

    monomerized superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPet-His

    Plasmid

    #14031

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Insert

    YPet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-hSOX2

    Plasmid

    #17218

    Purpose

    Retroviral expression of human SOX2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

    Plasmid

    #176541

    Purpose

    human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB omegaRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 Flag beta-1-adrenergic-receptor

    Plasmid

    #14698

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B1AR (ADRB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-ALK5 T204D

    Plasmid

    #80877

    Purpose

    mammalian expression of constitutively active ALK5 T204D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (constitutively active)

    Promoter

    CMV

    Available since

    Aug. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

20. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE-PIK3CA-E545K

    Plasmid

    #116485

    Purpose

    Lentiviral expression of PIK3CA E545K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E545K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST-FRT/T0-Flag-PALB2

    Plasmid

    #71114

    Purpose

    mammalian expession of FLAG-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pLVX-IRES-Hygro_RGECO1.2

    Plasmid

    #164592

    Purpose

    Stable integration and expression of RGECO1.2 in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    R-GECO1.2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSKBAD2

    Plasmid

    #15335

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Matrix-roGFP

    Plasmid

    #49437

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    Matrix-roGFP2
    

    Use

    Adenoviral

    Tags

    Cytochrome c oxidase subunit IV mitochondrial tar…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-SEP-GluA1 TKIT

    Plasmid

    #169441

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

    Plasmid

    #100146

    Purpose

    Expresses CPT1A

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    CPT1A (CPT1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hSyn1-eYFP

    Plasmid

    #117382

    Purpose

    An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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