We narrowed to 9,406 results for: Coli

1. pJZ002

   Plasmid

   #120231

   Purpose

   CRISPR-cas9 targeting in E. coli with ampicillin resistance

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH1-SaSrtA-KA

   Plasmid

   #64981

   Purpose

   Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108A

   Promoter

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPD3C

   Plasmid

   #86971

   Purpose

   Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

   Insert

   PCNA3 fused to phosphite dehydrogenase and P450cam
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A/A176R in PTDH, N106R in PCNA3, silent mutat…

   Promoter

   T7 promoter

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTYF-2xGfaABC1D-tTA

   Plasmid

   #19981

   Depositor

   Sergey Kasparov

   Article

   Liu et al J Gene Med. 2008 May . 10(5):583-92.

   Insert

   2xGfaABC1D-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pfMDH_H303A

   Plasmid

   #67730

   Purpose

   Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed histidine 303 to alanine

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pfMDH_D230A

   Plasmid

   #67725

   Purpose

   Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed aspartate 230 to alanine

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pfMDH_N191A

   Plasmid

   #67724

   Purpose

   Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 191 to alanine

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pfMDH_K295A

   Plasmid

   #67726

   Purpose

   Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed lysine 295 to alanine

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGHNC5

   Plasmid

   #24701

   Depositor

   George Allen

   Article

   Allen et al Plant Cell. 1996 May . 8(5):899-913.

   Insert

   Direct Repeats of Tobacco Rb7-6 MARs
   

   Use

   E. coli phagemid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEXndoE(E662Q)

    Plasmid

    #47716

    Purpose

    Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

    Depositor

    Mattias Collin, Vincent Fischetti

    Article

    Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

    Insert

    ndoE
    

    Use

    Tags

    GST-EndoE(E186Q) and Glutathione S-transferase

    Expression

    Bacterial

    Mutation

    Changed Glu-662 to Gln

    Promoter

    tac

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-P70a-Top-T~Bottom-T

    Plasmid

    #87315

    Purpose

    Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system (promoter control)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-4T1 human APC-2,3 (1362–1540)

    Plasmid

    #132631

    Purpose

    expresses human APC-2,3 (1362–1540) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

    Insert

    APC-2,3 (1362–1540)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB4C5_J23101-CocE

    Plasmid

    #128129

    Purpose

    Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRBBm76

    Plasmid

    #48117

    Purpose

    Shuttle vector E. coli/B.meg.

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    promoter sacB - gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHL 1454

    Plasmid

    #53019

    Purpose

    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    fimB
    

    Use

    Synthetic Biology

    Tags

    RBS(st7)

    Expression

    Bacterial

    Mutation

    Promoter

    pLlac0-1

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCDB326

    Plasmid

    #113670

    Purpose

    expresses Ulp1_R2 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R2
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-21a-GFP-Patronin CC 1288–1457

    Plasmid

    #59055

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid

    #82528

    Purpose

    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-84 with mutation of RKR70-72, K75, …

    Promoter

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHL 1916

    Plasmid

    #53023

    Purpose

    AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    fimB
    

    Use

    Synthetic Biology

    Tags

    Asp terminator and RBS(st7)

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pHL 1011

    Plasmid

    #52993

    Purpose

    pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    RBS (st7)
    

    Use

    Synthetic Biology

    Tags

    GFP and RBS-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    pLlac0-1 and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pHL1756

    Plasmid

    #53036

    Purpose

    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind and pConNoHindM12

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pRB7-6

    Plasmid

    #24700

    Depositor

    George Allen

    Article

    Allen et al Plant Cell. 1996 May . 8(5):899-913.

    Insert

    Tobacco Rb7 MAR (SAR)
    

    Use

    E. coli phagemid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pTrcHIS2-MtFAAH1

    Plasmid

    #243679

    Purpose

    Expresses Medicago truncatula FAAH1 isoform in E. coli.

    Depositor

    Kent Chapman

    Article

    Arias-Gaguancela et al Sci Rep. 2023 May 9;13(1):7486. doi: 10.

    Insert

    Medicago truncatula Fatty Acid Amide Hydrolase 1
    

    Use

    Tags

    c-myc, 6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTrcHIS2-MtFAAH2a

    Plasmid

    #243680

    Purpose

    Expresses Medicago truncatula FAAH2a isoform in E. coli.

    Depositor

    Kent Chapman

    Article

    Arias-Gaguancela et al Sci Rep. 2023 May 9;13(1):7486. doi: 10.

    Insert

    Medicago truncatula Fatty Acid Amide Hydrolase 2a
    

    Use

    Tags

    c-myc, 6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETDuet-larA-strep-larB

    Plasmid

    #232792

    Purpose

    Expression of L. plantarum larA-strep and larB genes in E. coli

    Depositor

    Robert Hausinger

    Article

    Nevarez et al mBio. 2024 Dec 16:e0340424. doi: 10.1128

    Inserts

    Lactate racemase
    Pyridinium-3,5-biscarboxylic acid mononucleotide synthase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-15b_T. maritima His-tRNase Z

    Plasmid

    #242436

    Purpose

    Expresses T. maritima tRNase Z in E. coli.

    Depositor

    Sebastian Maerkl

    Article

    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

    Insert

    tRNase Z
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJBL811

    Plasmid

    #204135

    Purpose

    Low-copy constitutively expressed sfGFP for E. coli

    Depositor

    Julius Lucks

    Article

    Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET-cfp29

    Plasmid

    #233651

    Purpose

    Mycobacterium tuberculosis Encapsulin CFP29 expression plasmid in E. coli

    Depositor

    Ye Gao

    Article

    Berger et al Commun Biol. 2025 Feb 15;8(1):245. doi:

    Insert

    Encapsulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MZB139

    Plasmid

    #217838

    Purpose

    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    10x His and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MZB164

    Plasmid

    #217842

    Purpose

    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    HaloTag
    

    Use

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOPINF HopF2

    Plasmid

    #228397

    Purpose

    Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopF2 (hopF2 Pseudomonas syringae pv. tomato)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSFDuet-larE-larC

    Plasmid

    #232793

    Purpose

    Expression of L. plantarum larE and LarC genes in E. coli

    Depositor

    Robert Hausinger

    Article

    Nevarez et al mBio. 2024 Dec 16:e0340424. doi: 10.1128

    Inserts

    Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
    Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-22b(+) pCopA sfgfp

    Plasmid

    #226374

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Use

    Tags

    6x HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    pCopA

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-22b(+) pCopA RBS30 sfgfp

    Plasmid

    #226371

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Use

    Tags

    6x HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA) and RBS30 regulation

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Halo-BASU-His

    Plasmid

    #218451

    Purpose

    E coli expression vector for Halo-BASU-His biotin ligase

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    HaloTag-BASU-His fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MZB216

    Plasmid

    #221766

    Purpose

    "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    rtxA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-AzaQ

    Plasmid

    #224429

    Purpose

    Express AzaQ in E. coli

    Depositor

    Emily Balskus

    Article

    Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

    Insert

    azaQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28b-M2

    Plasmid

    #225735

    Purpose

    To express M2 (219-399) in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 219-399

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET29_anti-MERS-Nanobody-VHH-1

    Plasmid

    #206890

    Purpose

    Express anti-MERS-Nanobody-VHH-1 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pET29_anti-MERS-Nanobody-VHH-55

    Plasmid

    #209417

    Purpose

    Express anti-MERS-Nanobody-VHH-55 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-55
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pET29_anti-MERS-Nanobody-VHH-83

    Plasmid

    #209418

    Purpose

    express anti-MERS-Nanobody-VHH-83 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pET29_anti-MERS-Nanobody-VHH-84

    Plasmid

    #209419

    Purpose

    Express anti-MERS-Nanobody-VHH-84 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pBad-MfnG

    Plasmid

    #191116

    Purpose

    Expresses MfnG in E. coli

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTrc-clbS_Bifido

    Plasmid

    #223359

    Purpose

    Encoding ClbS from Bifidobacterium longum subsp. infantis

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Bifidobacterium longum subsp. infantis (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTrc-clbS_Samsonia

    Plasmid

    #223357

    Purpose

    Encoding ClbS from Samsonia erythrinae DSM 16730

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Samsonia erythrinae DSM 16730 (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTrc-clbS_Gibsiella

    Plasmid

    #223356

    Purpose

    Encoding ClbS from Gibbsiella quercinecans DSM 25889

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    Encoding ClbS from Gibbsiella quercinecans DSM 25889 (Codon optimized for E coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET28b-M1

    Plasmid

    #225734

    Purpose

    To express M1 (13-193) in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 13-193

    Promoter

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJB68

    Plasmid

    #226315

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with Y to A CsoS2 MR1-7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pJB51

    Plasmid

    #226308

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-275AAA, VTG284-286AAA, VTG295-297AAA

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pJB48

    Plasmid

    #226307

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge