We narrowed to 14,123 results for: cas9 genes
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sg2.02 16x(MS2) MUC4.1
Plasmid#101154PurposegRNA with 16 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TGM2
Plasmid#69239PurposeU6 driven SpCas9 sgRNA expression for TGM2 siteDepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA-ura-HYB
Plasmid#64330Purposeura3 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of ura3 deletion gRNA cassette
ExpressionYeastAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C.NR
Plasmid#185672PurposeExpresses nuclease active eNme2-C.NR in mammalian cellsDepositorInserteNme2-C.NR nuclease
UseCRISPRExpressionMammalianMutationeNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…Available SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGreenGate-M (pGGG-M)
Plasmid#165422PurposeA Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).TypeEmpty backboneUseSynthetic Biology; Level 2 golden gate (moclo) du…PromoterContain GG cassette and MCS with LacZ for blue/wh…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1-Scr
Plasmid#133042PurposeSiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
ExpressionYeastAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRISPseq-BFP-backbone
Plasmid#85707PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
SauriBE4max
Plasmid#135969PurposeExpresses AncBE4max and nSauriCas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-PuroR
Plasmid#196962PurposeExpresses PE2 with puromycin resistanceDepositorInsertPE2-P2A-Puro
ExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
SauriABEmax
Plasmid#135968PurposeExpresses ABEmax and nSauriCas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA-leu-HYB
Plasmid#64332Purposeleu2 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of leu2 deletion gRNA cassette
ExpressionYeastAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL12009
Plasmid#68257PurposeLevel 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)DepositorInsertPromoter and 5UTR Ubiquitin (Zea mays)
UseSynthetic BiologyAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRISPseq-mCherry-backbone
Plasmid#85708PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH47802-p35S::tdTomato-ER::tNOS
Plasmid#160220PurposetdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverseDepositorInsertp2x35S::TdTomato-ER::tNOS
UseSynthetic Biology; Moclo toolkit level 1 acceptor…ExpressionPlantAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
gRNA-his-HYB
Plasmid#64333Purposehis3 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of his3 deletion gRNA cassette
ExpressionYeastAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdMCP-mCherry
Plasmid#99909PurposeFor the expression of NLS-tdMCP-mCherryDepositorInsertNLS-tdMCP-mCherry
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
sg14x(MS2) MUC4.1
Plasmid#101153PurposegRNA with 14 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdPCP-EGFP
Plasmid#99910PurposeFor the expression of NLS-tPMCP-EGFPDepositorInsertNLS-tdPCP-EGFP
UseLentiviralExpressionMammalianPromoterTRE4GAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AM77_pU6.Sa-gRNA.CLYBL
Plasmid#199237PurposeExpression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. aureus gRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNAAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-EGFP
Plasmid#100603PurposeDonor scaffold with EGFP positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsEGFP-T2A-Puro and tagBFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
gh19
Plasmid#106703Purposeexpression of gRNA targeting GALNT19DepositorInsertGALNT19
ExpressionMammalianAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pPV-H1-ccdB-mEF1α-RiH
Plasmid#100598PurposepiggyBac vector for sgRNA cloningDepositorTypeEmpty backboneUsePiggybac vectorExpressionMammalianPromoterHuman H1 PolIII promoter, human FTH1 promoter, mo…Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-dTOMATO
Plasmid#100604PurposeDonor scafold with dTOMATO positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsdTOMATO-T2A-Puro and tagBFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEJS433-pCSDest2-AcrIIC1Nme
Plasmid#85679PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC1Nme
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-Gateway
Plasmid#114011Purpose3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNADepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2.0 (PP7) Locus#2
Plasmid#101155PurposegRNA with two PCP binding sitesDepositorInsertLocus #2(tandem repeat)
UseCRISPR and LentiviralPromoterhU6Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
SGL40C-H1.EFS.RFP657
Plasmid#69148PurposeAdvanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
Available SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL80036
Plasmid#68259PurposeLevel 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)DepositorInsertCoding sequence, hygromycin phophotransferase II (Escherichia coli)
UseSynthetic BiologyAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pEF-XMAS-2xStop
Plasmid#164412PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianPromoterEF1-alphaAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEL666
Plasmid#196814PurposeLrCas9 genome editing plasmid in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionPlantPromoterZmUbiAvailable SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS436-pCSDest2-AcrIIC2Nme
Plasmid#85712PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC2Nme
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
sg2.02 MUC4.1
Plasmid#101152PurposegRNA with two MCP binding sitesDepositorAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_MYOD1
Plasmid#64136PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.DepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_MYOD1
Plasmid#64137PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.DepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-2x
Plasmid#164414PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA4_MYOD1
Plasmid#64139PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-NT1-6-gix-6-NT1
Plasmid#81204Purposecontains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmidDepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_MYOD1
Plasmid#64138PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS443-pCSDest2-AcrIIC3Nme
Plasmid#85713PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC3Nme
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-SAM
Plasmid#78905PurposeVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceAug. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJM149
Plasmid#134168PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC E118A R129A, E. coli codon optimized, CRISPR…Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM147
Plasmid#134167PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
