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mTagRFP-T-Mito-7

Plasmid

#58023

Purpose

Localization: Mitochondria, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Mito (COX8A Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pNestin-EGFP

Plasmid

#38777

Depositor

Wei Cui

Article

Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

Insert

nestin 2nd intron enhancer with TK mini promoter (NES Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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tdTomato-Golgi-7

Plasmid

#58100

Purpose

Localization: Golgi, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Golgi (B4GALT1 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
DsRed2-ER-5

Plasmid

#55836

Purpose

Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

ER (CALR Human)

Use

Tags

DsRed2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEmerald-LaminB1-10

Plasmid

#54140

Purpose

Localization: Nuclear Envelope, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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mPlum-Mito-3

Plasmid

#55988

Purpose

Localization: Mitochondria, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Mito (COX8A Human)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-ER-14

Plasmid

#56432

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

ER (CALR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mOrange2-Actin-7

Plasmid

#57943

Purpose

Localization: Actin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Actin (ACTB Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
mCitrine-VE-Cadherin-N-10

Plasmid

#56319

Purpose

Localization: Tight Junctions, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

VE-Cadherin (CDH5 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

I503T in VE-Cadherin

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFRT-TODestFLAGHA_RBPMS_R38E

Plasmid

#65665

Purpose

FLAG-HA expression vector with RBPMS carrying mutation R38E

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamic acid

Promoter

CMV, 2x Tet

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38Q

Plasmid

#65094

Purpose

Destination vector with RBPMS with R38Q mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamine

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_R38A_E39A

Plasmid

#65664

Purpose

FLAG-HA expression vector with RBPMS carrying mutation R38A, E39A

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to alanine, and glutamic acid…

Promoter

CMV, 2x Tet

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTurboRFP-Farnesyl-5

Plasmid

#58057

Purpose

Localization: Plasma Membrane, Excitation: 553, Emission: 576

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Farnesyl (HRAS Human)

Use

Tags

tdTurboRFP

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFRT-TODestFLAGHA_RBPMS2_HindIII

Plasmid

#65666

Purpose

FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR

Plasmid

#65091

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed sequence to render plasmid resistant to s…

Promoter

CMV, 2x Tet

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestMYC_RBPMS_KDR

Plasmid

#65667

Purpose

MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

introduced HindIII site to exchange FLAG-HA tag w…

Promoter

CMV, 2x Tet

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR_K100E

Plasmid

#65093

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 100 to glutamic acid (KDR backgrou…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR_F65A

Plasmid

#65092

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

change phenylalanine 65 to alanine (KDR backbone)

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_K36E_R38E

Plasmid

#65095

Purpose

Destination vector with RBPMS with K36E/R38E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

change lysine 36 to glutamic acid and arginine 38…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge