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We narrowed to 21,484 results for: SPR

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Showing: 5281 - 5320 of 21484 results

  2. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164559-plasmid-map-sequence-id-330205

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  10. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Article
    Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    218775-plasmid-map-sequence-id-443448

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  11. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139991-plasmid-map-sequence-id-275523

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  12. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  16. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

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  17. pCAG_iPE-N

    Plasmid
    #214734
    Purpose
    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-N_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214734-plasmid-map-sequence-id-453897

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  22. pNR4A1.1.0-gDNA

    Plasmid
    #112391
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1
    Depositor
    Kevin White
    Insert
    NR4A1 (NR4A1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112391-plasmid-map-sequence-id-230237

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  27. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  28. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid
    #187953
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187953-plasmid-map-sequence-id-373487

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  29. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid
    #207404
    Purpose
    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]
    Depositor
    Alexandros Poulopoulos
    Article
    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10
    Insert
    Cas9-RC
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP-2A
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    207404-plasmid-map-sequence-id-413204

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  40. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

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Showing: 5281 - 5320 of 21484 results