4,136 results

1. pCAGMKOSiE

   Plasmid

   #20865

   Depositor

   Keisuke Kaji

   Article

   Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

   Insert

   myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. RIP Cre-ER (DM#265)

   Plasmid

   #15029

   Depositor

   Douglas Melton

   Article

   Dor et al Nature. 2004 May 6. 429(6987):41-6.

   Insert

   Rat insulin promoter (RIP) (Ins2 Rat)
   

   Use

   Cre/Lox; For making transgenic constructs

   Tags

   Cre-ER (TM)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP2443

   Plasmid

   #72252

   Purpose

   Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRsetB-his7-Perceval

   Plasmid

   #20336

   Depositor

   Gary Yellen

   Article

   Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

   Insert

   Perceval (fluorescent ATP:ADP sensor)
   

   Use

   Tags

   his(7)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC-GoldyTALEN

   Plasmid

   #38143

   Purpose

   destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

   Depositor

   Daniel Carlson

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Tags

   AcV5 and FokI homodimer

   Expression

   Mammalian

   Mutation

   Truncate at N and C terminous

   Promoter

   miniCaggs

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 barr2 HA

   Plasmid

   #14692

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   beta-arrestin 2 (Arrb2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-ENTR A (696-6)

   Plasmid

   #17427

   Purpose

   Gateway entry vector with EF1-a promoter, V5-His C-term, frame A

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAlbumin

   Plasmid

   #22037

   Depositor

   Didier Trono

   Insert

   partial sequence of human albumine
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-hDKK1-flag

   Plasmid

   #15494

   Depositor

   Sergei Sokol

   Article

   Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

10. SRa-HA-SUMO2 (Sentrin 3)

    Plasmid

    #17360

    Purpose

    Mammalian expression of human SUMO2 with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXs-hNANOG (Plath)

    Plasmid

    #18115

    Depositor

    Kathrin Plath

    Article

    Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

    Insert

    Nanog homeobox (NANOG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA 3.1(-) mouse C/EBP delta

    Plasmid

    #12559

    Depositor

    Peter Johnson

    Article

    McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

    Insert

    CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S2G introduced during cloning

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. FCK-Halo-GFP

    Plasmid

    #14750

    Purpose

    Lentiviral expression of Halo-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al PLoS One. 2007 Mar 21;2(3):e299.

    Insert

    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N. pharaonis halorhodopsin is mammalian codon-opt…

    Promoter

    CaMKIIa

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAd-Track Flag-SIRT1

    Plasmid

    #8438

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-CRY2-CreN

    Plasmid

    #26888

    Purpose

    Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2-CreN (cre, CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 19-104 of Cre

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHB9-EGFP (Hb9 promoter) [TJ#96]

    Plasmid

    #16275

    Depositor

    Thomas Jessell

    Article

    Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

    Insert

    HB9 promoter
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5 TGF beta type I receptor Flag

    Plasmid

    #14831

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Stat3 S727A pRc/CMV

    Plasmid

    #8708

    Depositor

    Jim Darnell

    Article

    Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

    Insert

    Stat3 S727A (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S727A (exhibits o serine phosphorylation either c…

    Promoter

    Available since

    Aug. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

    Plasmid

    #19326

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::twk-18(cn110)cDNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMLM292

    Plasmid

    #21873

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC57 APEX

    Plasmid

    #40306

    Purpose

    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APEX (Synthetic)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    K14D, W41F, E112K (relative to wild-type)

    Promoter

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMLM290

    Plasmid

    #21872

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGFP-Rab8A[T22N]

    Plasmid

    #24899

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[T22N]
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 22 to Asparagine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pB1H2w2-zif268

    Plasmid

    #18045

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Zif268 is fused to the omega subunit of RNA polym…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Kunihiko Naito, Wataru Fujii

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge