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Showing: 6701 - 6750 of 12238 results

  4. pCEP4-191084-QES.i06.c07

    Plasmid
    #123239
    Purpose
    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (191084 B7-19) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations P124D, …
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123239-plasmid-map-sequence-id-235191

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  5. pCEP4-001428-QES.c10.L544Y-754*

    Plasmid
    #123253
    Purpose
    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (001428) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123253-plasmid-map-sequence-id-235211

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  6. pCEP4-ZM197M-QES.c10-754*

    Plasmid
    #123222
    Purpose
    Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (ZM197M) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123222-plasmid-map-sequence-id-235165

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  7. pCEP4-Q842-QES.i04.c05

    Plasmid
    #123233
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations P124D, …
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123233-plasmid-map-sequence-id-235181

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  8. pCEP4-Q769-QES.i03.c04

    Plasmid
    #123229
    Purpose
    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q769.d22) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations A200E, …
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123229-plasmid-map-sequence-id-235175

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  9. pCEP4-H031-QES.i07.c09

    Plasmid
    #123271
    Purpose
    Mammalian expression plasmid for Env from the H031 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (H031) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations S49D, F…
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123271-plasmid-map-sequence-id-235234

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  10. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid
    #123269
    Purpose
    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    SHIV327C-Hu A10 Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations V208M, …
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123269-plasmid-map-sequence-id-235232

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  11. pCEP4-B41gp160-V200E-754*

    Plasmid
    #123244
    Purpose
    Mammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (B41) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123244-plasmid-map-sequence-id-235194

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  12. pCEP4-25711-QES.c11-754*

    Plasmid
    #123218
    Purpose
    Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (25711) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    123218-plasmid-map-sequence-id-235160

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  13. pCEP4-Q842gp160-QES.04

    Plasmid
    #100933
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Use
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; mutations P124D;F…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100933-plasmid-map-sequence-id-197641

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  14. pCEP4-Q842gp160-QES.i04.I181L

    Plasmid
    #111837
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Use
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; mutations P124D;I…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111837-plasmid-map-sequence-id-210164

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  15. pCEP4-Q769gp160-QES.i03.V255M

    Plasmid
    #111838
    Purpose
    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q769.d22) Env
    Use
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; mutations A200E;F…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111838-plasmid-map-sequence-id-210171

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  16. IO801: pMVP (L5-L4) 3x FLAG epitope tag

    Plasmid
    #121702
    Purpose
    pMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x FLAG epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121702-plasmid-map-sequence-id-239011

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  17. pCEP4-HA-Flag-natT1R2 d835

    Plasmid
    #113959
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-834
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113959-plasmid-map-sequence-id-215288

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  18. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid
    #113958
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-839, R837G and R838G substitution
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113958-plasmid-map-sequence-id-215287

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  26. pET-30a(+)-C-SH2+IA

    Plasmid
    #111418
    Purpose
    express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p
    Insert
    murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111418-plasmid-map-sequence-id-215713

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  28. pMflT-o3

    Plasmid
    #101311
    Purpose
    Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101311-plasmid-map-sequence-id-198141

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  29. PatoM-OG241

    Plasmid
    #72842
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoM
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoM
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72842-plasmid-map-sequence-id-133606

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  30. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

    Plasmid
    #70195
    Purpose
    Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector
    Depositor
    Richard Murray
    Article
    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.
    Insert
    tetR-deGFP fusion
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    70195-plasmid-map-sequence-id-125453

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  31. pUAST-HA, Pbl-A DH domain

    Plasmid
    #69794
    Purpose
    Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble DH domain
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    Contains only the Pebble DH domain
    Promoter
    hsp70
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

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  33. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59894-plasmid-map-sequence-id-108252

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  34. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  35. pLS-mP-Luc

    Plasmid
    #106253
    Purpose
    Luciferase lentivirus based enhancer assay vector
    Depositor
    Nadav Ahituv
    Insert
    Luciferase
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Derived from pGL4.23
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106253-plasmid-map-sequence-id-208513

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  36. Lentiviral nontargeting shRNA GFP

    Plasmid
    #155285
    Purpose
    Lentiviral expression of nontargeting shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155285-plasmid-map-sequence-id-311904

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  37. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

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  38. MZB223

    Plasmid
    #228491
    Purpose
    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228491-plasmid-map-sequence-id-455392

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  39. pGGD_RT_biChromium

    Plasmid
    #136974
    Purpose
    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide
    Depositor
    Klaus Theres
    Insert
    P2A sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136974-plasmid-map-sequence-id-270595

    Enlarge

  40. Dox nontargeting shRNA GFP

    Plasmid
    #155290
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155290-plasmid-map-sequence-id-311906

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  41. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    1732-plasmid-map-sequence-id-326991

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  42. Dox nontargeting shRNA mKate

    Plasmid
    #155292
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155292-plasmid-map-sequence-id-310436

    Enlarge

  43. Lentiviral nontargeting shRNA RFP i670

    Plasmid
    #155287
    Purpose
    Lentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155287-plasmid-map-sequence-id-310003

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  44. pCfb12567

    Plasmid
    #219927
    Purpose
    PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter CYC
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    219927-plasmid-map-sequence-id-460324

    Enlarge

  45. pCfb12566

    Plasmid
    #219926
    Purpose
    PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter DGA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    219926-plasmid-map-sequence-id-460318

    Enlarge

  46. pCfb12622

    Plasmid
    #219929
    Purpose
    PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter ACT
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219929-plasmid-map-sequence-id-436186

    Enlarge

  47. pCfb12562

    Plasmid
    #219923
    Purpose
    PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter GPD
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219923-plasmid-map-sequence-id-460320

    Enlarge

  48. pCfb13186

    Plasmid
    #219924
    Purpose
    PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter TEF
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219924-plasmid-map-sequence-id-436038

    Enlarge

  49. pCfb12620

    Plasmid
    #219928
    Purpose
    PrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter TPI
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    219928-plasmid-map-sequence-id-435947

    Enlarge

  50. pCfb12561

    Plasmid
    #219922
    Purpose
    PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter PGM
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219922-plasmid-map-sequence-id-435639

    Enlarge

Showing: 6701 - 6750 of 12238 results