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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,591 results for: EPO

Showing: 6701 - 6750 of 9591 results

  1. pITGFP89

    Plasmid
    #83560
    Purpose
    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Insert
    TEF1 promoter -yEGFP-CLN2PEST
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83560-plasmid-map-sequence-id-159500

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  3. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid
    #68432
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68432-plasmid-map-sequence-id-125560

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  10. cycLuc_SbMVs

    Plasmid
    #119209
    Purpose
    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with SbMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119209-plasmid-map-sequence-id-231830

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  11. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

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  17. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsubDD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84633-plasmid-map-sequence-id-176152

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  18. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid
    #169552
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-NanoLuc Luciferase - mTagBFP fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169552-plasmid-map-sequence-id-364979

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  19. miR-34aM3

    Plasmid
    #50829
    Purpose
    luciferase reporter for human miR-34a
    Depositor
    Xueqing Xu, Yun Bai
    Article
    Li et al BMC Mol Biol. 2012 Jan 31;13:4.
    Insert
    miR-34A promoter M3 (MIR34A Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    third NF-kB binding site mutated (-149)
    Promoter
    miR34a
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. miR-34aM1

    Plasmid
    #50827
    Purpose
    luciferase reporter for human miR-34a
    Depositor
    Xueqing Xu, Yun Bai
    Article
    Li et al BMC Mol Biol. 2012 Jan 31;13:4.
    Insert
    miR-34A promoter M1 (MIR34A Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    first NF-kB binding site mutated, (-539)
    Promoter
    miR34a
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pSB1A3 EcoRI Methylase-AmilCP

    Plasmid
    #85166
    Purpose
    For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI Methylase-AmilCP
    Use
    Tags
    AmilCP
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. spa-GCaMP6f

    Plasmid
    #67558
    Purpose
    photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Tags
    HIS-tag
    Expression
    Mammalian
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    CMV
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

    Plasmid
    #182349
    Purpose
    mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182349-plasmid-map-sequence-id-361661

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  30. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Judith Kimble
    Article
    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:
    Insert
    his-58, eGFP, mCherry
    Use
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171845-plasmid-map-sequence-id-337181

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  33. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

    Plasmid
    #158190
    Purpose
    Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    DYRK3-V5-miniTurbo (DYRK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    R293H- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    158190-plasmid-map-sequence-id-328919

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  37. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

    Plasmid
    #162918
    Purpose
    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162918-plasmid-map-sequence-id-330526

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  40. pRSET-spa-GCaMP6f

    Plasmid
    #67563
    Purpose
    bacterial expression of a photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Tags
    HIS-tag
    Expression
    Bacterial
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. RAB14 3'UTR D1D2

    Plasmid
    #61492
    Purpose
    luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

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  50. p3x-Flag Gli3 37R3-2

    Plasmid
    #120305
    Purpose
    Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C
    Depositor
    David Liu
    Article
    Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi
    Insert
    Intein 37R3-2, Gli3
    Use
    Synthetic Biology
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Contains mutations as reported in 2011 Chemistry …
    Promoter
    CMV
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    120305-plasmid-map-sequence-id-234346

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Showing: 6701 - 6750 of 9591 results