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We narrowed to 18,759 results for: nls

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Showing: 5481 - 5520 of 18759 results

  1. pMXs-IP-mCherry-TOSI

    Plasmid
    #172496
    Purpose
    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Publication
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    PDCD4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    Mcherry
    Expression
    Mammalian
    Promoter
    MLV LTR
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172496-plasmid-map-sequence-id-338550

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  2. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Promoter
    pRhaBAD
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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  6. pZFN-AAVS1-KKR

    Plasmid
    #159298
    Purpose
    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    ZFN targeting the AAVS1 locus
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159298-plasmid-map-sequence-id-311594

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  16. pAC1749

    Plasmid
    #127983
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127983-plasmid-map-sequence-id-254502

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  20. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122183-plasmid-map-sequence-id-241282

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  22. pDHt/sk-PC

    Plasmid
    #92125
    Purpose
    Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR; Trichoderma reesei expression
    Promoter
    Ppdc
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92125-plasmid-map-sequence-id-215731

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  25. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid
    #102851
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3X Flag and NLS
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102851-plasmid-map-sequence-id-198995

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  26. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98998-plasmid-map-sequence-id-190366

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  29. FUCas9GFP

    Plasmid
    #85555
    Purpose
    Expresses Cas9 with green fluorescent reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyogenes Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    hUBC
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85555-plasmid-map-sequence-id-169910

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  30. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84244-plasmid-map-sequence-id-160887

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  39. pNSEN-d2

    Plasmid
    #59763
    Purpose
    Expresses nuclear EGFP and Tomato in mammalian cells
    Depositor
    Jerry Crabtree
    Publication
    Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    d2-EGFP
    Tomato
    Tags
    3X NLS and PEST
    Expression
    Mammalian
    Promoter
    CMV promoter and SV40 promoter
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59763-plasmid-map-sequence-id-94069

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  40. pMB62

    Plasmid
    #47944
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP
    Expression
    Worm
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    47944-plasmid-map-sequence-id-68804

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Showing: 5481 - 5520 of 18759 results