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We narrowed to 21,484 results for: SPR

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Showing: 5521 - 5560 of 21484 results

  1. pX330 _CRISPIE donor B10

    Plasmid
    #172851
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172851-plasmid-map-sequence-id-341020

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  2. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195294-plasmid-map-sequence-id-385588

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  3. pJAT77

    Plasmid
    #204295
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204295-plasmid-map-sequence-id-406178

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  6. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Article
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    131124-plasmid-map-sequence-id-257454

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  7. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108860-plasmid-map-sequence-id-209932

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  27. NG-ABE-NW

    Plasmid
    #240622
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA8e(N108Q, M151C, Q154Y)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e(N108Q, M151C, Q154Y), Cas9 (D10A, L1111R, …
    Available Since
    July 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240622-plasmid-map-sequence-id-480855

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  28. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

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  29. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  34. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  40. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102890
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102890-plasmid-map-sequence-id-198249

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Showing: 5521 - 5560 of 21484 results