159,617 results

1. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-hSyn1-GFP

   Plasmid

   #177810

   Purpose

   Encodes a GFP driven by human synapsin I promoter

   Depositor

   Christopher Grigsby

   Article

   Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

   Insert

   CopGFP inserted behind human synapsin I promoter (SYN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Syn1

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1-muscle Amphiphysin 2

   Plasmid

   #22213

   Depositor

   Pietro De Camilli

   Article

   Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

   Insert

   Amphiphysin II (BIN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pR BirA

   Plasmid

   #113640

   Purpose

   Insect cell expression vector for BirA Biotin Ligase

   Depositor

   James Moon

   Article

   Moon et al Methods Mol Biol. 2018;1799:165-181. doi

   Insert

   BirA Biotin Ligase (birA )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-puro_GCaMP6s

   Plasmid

   #164589

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAIDA1

   Plasmid

   #79180

   Purpose

   A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

   Depositor

   Gen Larsson

   Article

   Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

   Insert

   Adhesin Involved in Diffuse Adherence
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR E2 HSPter-35Sp-mito

   Plasmid

   #158727

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   A terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-BDNF-Myc

   Plasmid

   #124279

   Purpose

   Express Myc-tagged brain-derived neurotrophic factor (BDNF)

   Depositor

   Baoji Xu

   Article

   Xie et al Cell Metab. 2019 Jan 11. pii: S1550-4131

   Insert

   BDNF (Bdnf Mouse)
   

   Use

   AAV

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. STIM1-EGFP

   Plasmid

   #210370

   Purpose

   Expresses STIM1 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+) mCherry

    Plasmid

    #176301

    Purpose

    Expresses mCherry mRNA from CMV promoter

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR E3 pR6A L3-L2

    Plasmid

    #158733

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR E3 pR7A L3-L2

    Plasmid

    #158734

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR E1 pF7A L1-L4

    Plasmid

    #158730

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR E1 pF6A L1-L4

    Plasmid

    #158729

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCVpuro-Cre

    Plasmid

    #34564

    Purpose

    Retroviral expression of Cre

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. ORFtag_TetR-SD_Frame1

    Plasmid

    #220981

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. COVID-SARS2 NSP13

    Plasmid

    #159614

    Purpose

    Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

    Depositor

    Opher Gileadi

    Article

    Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

    Insert

    Covid-SARS2 Nsp13
    

    Use

    Tags

    His6-Zb-TEV

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    T7 - LacO

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-3xFLAG-BioID2-MCS

    Plasmid

    #186811

    Purpose

    CAG promoter-driven expression of BioID2-fused protein (N terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-BioID2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLifeAct_mScarlet_N1

    Plasmid

    #85054

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

    Plasmid

    #205631

    Purpose

    Expression of TnpBmax in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB optimized for mammalian expression
    

    Use

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780)

    Plasmid

    #214848

    Purpose

    Expression of iCre(R297T) in striatal cholinergic neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R297T

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF2027

    Plasmid

    #143994

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NPAS2 (NPAS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LZRS-DNMT1

    Plasmid

    #24952

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA5/FRT/TO_H2B_HaloTag7

    Plasmid

    #169329

    Purpose

    Expression of H2B-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 (H2BC21 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG425GPD-ccdB

    Plasmid

    #14154

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNat-AID*-9myc

    Plasmid

    #99523

    Purpose

    C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKD3

    Plasmid

    #45604

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-cat-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    R6K

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSBbi-pur H-2Kb

    Plasmid

    #111623

    Purpose

    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

    Depositor

    Jon Yewdell

    Insert

    H2Kb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pY094

    Plasmid

    #84743

    Purpose

    Expresses huAsCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    GFP
    

    Use

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CAG-FR-GECO1c

    Plasmid

    #163682

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1c

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1+_Lambda-N-HA-Peptide

    Plasmid

    #92005

    Purpose

    Expresses Lambda-N-HA peptide

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Lambda-N-HA peptide
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGR481

    Plasmid

    #213783

    Purpose

    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

    Depositor

    Chang Liu

    Insert

    ura3*
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid

    #52093

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR223-MERTK

    Plasmid

    #23900

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MERTK (MERTK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPB-CAG-rtTA3G-IH

    Plasmid

    #214118

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    trTA3G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET His6 MBP TEV LIC cloning vector (2M-T)

    Plasmid

    #29708

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MGAT2-RpHLuorin2

    Plasmid

    #171718

    Purpose

    Cis-/medial-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Human, Synthetic)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge