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167,150 results

Showing: 5601 - 5640 of 167150 results

  1. pCLX-PGK-hTERT

    Plasmid
    #114315
    Purpose
    Constitutive expression of the hTert protein
    Depositor
    Patrick Salmon
    Insert
    hTERT (TERT Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114315-plasmid-map-sequence-id-218852

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  3. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

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  6. pMOD_C2515

    Plasmid
    #91083
    Purpose
    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    AtU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91083-plasmid-map-sequence-id-182047

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  7. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

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  8. pCMV-2E2-HA-mCh

    Plasmid
    #129596
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129596-plasmid-map-sequence-id-255026

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  9. pcDNA3-CCL21-V5

    Plasmid
    #11609
    Depositor
    Steven Johnson
    Insert
    chemokine (C-C motif) ligand 21 (CCL21 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  14. TFORF1125

    Plasmid
    #143093
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ENO1 (ENO1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143093-plasmid-map-sequence-id-399018

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  17. pTA7002-avrPto

    Plasmid
    #49156
    Purpose
    Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto
    Depositor
    Sheng Yang He
    Article
    Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100
    Insert
    avrPto
    Use
    Plant transformation binary vector
    Promoter
    dexamethasone inducible
    Available Since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Expression
    Mammalian
    Promoter
    Tet-On 3G Bidirectional
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  21. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  23. 803 pGEX2T PTEN

    Plasmid
    #10784
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  28. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)

    Plasmid
    #163505
    Purpose
    DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Promoter
    minBetaGlobin
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163505-plasmid-map-sequence-id-325646

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  36. mCherry-ATG14-C-18

    Plasmid
    #54989
    Purpose
    Localization: Membrane Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ATG14 (ATG14 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. TFORF0985

    Plasmid
    #142249
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF4 (KLF4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    142249-plasmid-map-sequence-id-385602

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  39. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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Showing: 5601 - 5640 of 167150 results