We narrowed to 58,797 results for: ran

1. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

   Plasmid

   #113890

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. amilGFP chromoprotein promoter-less

   Plasmid

   #118006

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOP/SSAT

   Plasmid

   #140291

   Purpose

   Expresses human SSAT in bacterial cells

   Depositor

   Saverio Bettuzzi, Gaetano Marverti

   Article

   Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

   Insert

   SSAT1 (SAT1 Human)
   

   Expression

   Bacterial

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1-1180

   Plasmid

   #13265

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Expression

   Bacterial

   Mutation

   Truncated after RSLE region

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCW57.1_V5_mNeonGreen_FLAG_C

   Plasmid

   #218073

   Purpose

   RESOLUTE cloning plasmid

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-mNeonGreen-V5

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE MDER

   Plasmid

   #13494

   Depositor

   Stephen Tapscott

   Article

   Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

   Insert

   MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   full length MyoD was fused to aa282-595 of Esr1. …

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-MCK1.35lux

   Plasmid

   #53368

   Purpose

   expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

   Depositor

   Josephine Nalbantoglu

   Article

   Larochelle et al Gene Ther. 1997 May;4(5):465-72.

   Insert

   muscle creatine kinase promoter/enhancer 1.35kb
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET15b-sfGO-Matryoshka-LE-LP

   Plasmid

   #100018

   Purpose

   Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpsfGFP-LSSmOrange
   

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF005-sgRNA-shuffle-in

   Plasmid

   #104441

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    U6 promoter

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT3024

    Plasmid

    #122542

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_mt::CemOrange2
    

    Tags

    MLSLRQSIRFFKPATRTLCSSRTLL

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRB-mCherry-EEA1

    Plasmid

    #222419

    Purpose

    To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    EEA1 (EEA1 Human)
    

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.Flag.HERC2-HECT-WT.6xHis

    Plasmid

    #39227

    Depositor

    Aziz Sancar

    Article

    Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

    Insert

    HERC2 HECT Domain (HERC2 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

    Plasmid

    #120269

    Purpose

    Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    eSyn-DIO-TVA950:YFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEYFP-hDF-C2A

    Plasmid

    #72582

    Purpose

    human dysferlin C2A domain tagged with YFP

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin C2A domain (DYSF Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C2A domain

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. L1dRSLE

    Plasmid

    #13266

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    RSLE region removed

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

18. tsPurple chromoprotein promoter-less

    Plasmid

    #118007

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-ETV6-ETS

    Plasmid

    #111180

    Purpose

    Minimal ETS domain of human ETV6 (TEL)

    Depositor

    Gregory Poon

    Article

    Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

    Insert

    ETV6 ETS domain (ETV6 Mouse, Bovine, Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET a MatryoshCaMP6s

    Plasmid

    #100019

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMuLE ENTR 7SK-miR-30 L1-L4

    Plasmid

    #62180

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDGB1alpha2_SulR

    Plasmid

    #186425

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mRuby2-H2B-C-10

    Plasmid

    #55898

    Purpose

    Localization: Nucleus/Histones, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    H2B (H2BC11 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pMS1: pHelper(AvCAST)_AvPSP1

    Plasmid

    #168133

    Purpose

    Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC5-dual-dCas9VP48-sgTetO

    Plasmid

    #48237

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48 and sgTetO
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2

    Plasmid

    #62168

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLINE1-scFvFc

    Plasmid

    #206020

    Purpose

    An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pU6-sgGAL4-4

    Plasmid

    #46916

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-4
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. DNM2 delta PRD

    Plasmid

    #168275

    Purpose

    Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

    Depositor

    Cheng-han Yu

    Article

    Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

    Insert

    DNM2 (DNM2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa 1-742

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV-EF1a-Tmx4-V5

    Plasmid

    #120236

    Purpose

    Expresses V5-tagged Tmx4 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3HA-ErbB4CTF

    Plasmid

    #17803

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292

    Available Since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

36. plc242-GFP-ITFG2

    Plasmid

    #87049

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBa.GFP-AP1/beta1

    Plasmid

    #218802

    Purpose

    Mammalian expression o fhuman AP1 b1

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    AP1 b1 (AP1B1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Chicken Beta actin

    Available Since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. promoter J23110 (upstream of RBS, mRFP1)

    Plasmid

    #117852

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    medium-high promoter, RBS, mRFP1
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-ROR1-V5/HIS

    Plasmid

    #205607

    Purpose

    Expression of human ROR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR1 receptor tyrosine kinase (ROR1 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TMEM-bio-His

    Plasmid

    #51856

    Purpose

    Expresses full-length Transmembrane protein 109 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMEM (TMEM109 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47454

    Purpose

    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    CRY2PHR-NLS-SID4X
    

    Use

    AAV

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSVE Grb2 myc 49L/203R

    Plasmid

    #26087

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 49L/203R (GRB2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    49L/203R

    Available Since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

43. pcDNA3.1 MYC dLZ

    Plasmid

    #176048

    Purpose

    Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 414-433

    Promoter

    CMV

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRK5-MYC-ITFG2

    Plasmid

    #87046

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMuLE ENTR CMV CreERT2 R4-R3

    Plasmid

    #62173

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    CreERT2
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSL0373 (His-MBP-TniQ)

    Plasmid

    #130638

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

    Plasmid

    #168135

    Purpose

    Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. mRuby2-H2B-6

    Plasmid

    #55897

    Purpose

    Localization: Nucleus/Histones, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    H2B (H2BC11 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

49. pET24a-VHH-tev-APEX2

    Plasmid

    #117751

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Promoter

    UBC

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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