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Showing: 7201 - 7250 of 12236 results

  1. p426_Cas9_gRNA-ARS1014a

    Plasmid
    #87396
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87396-plasmid-map-sequence-id-193439

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  3. pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid
    #105202
    Purpose
    Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    105202-plasmid-map-sequence-id-201273

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  4. p426_Cas9_gRNA-YOLCd1b

    Plasmid
    #87401
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YOLCd1b sequence GACTAGTTAAGCGAGCATGT in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YOLCd1b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87401-plasmid-map-sequence-id-193444

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  5. p426_Cas9_gRNA-CAN1y

    Plasmid
    #87391
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87391-plasmid-map-sequence-id-193434

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  6. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49069-plasmid-map-sequence-id-72196

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  16. pETcon-pGAL1-Aga2-Amyloid β42-c_myc

    Plasmid
    #225223
    Purpose
    Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360
    Inserts
    Amyloid β42
    Aga2 (AGA2 Budding Yeast)
    Use
    Tags
    HA Tag and c-myc Tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225223-plasmid-map-sequence-id-460494

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  18. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

    Plasmid
    #225222
    Purpose
    Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360
    Inserts
    α-Synuclein
    Aga2
    Use
    Tags
    GS linker, HA Tag, and c-myc Tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225222-plasmid-map-sequence-id-458386

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Showing: 7201 - 7250 of 12236 results