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We narrowed to 10,761 results for: T7

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Showing: 5801 - 5840 of 10761 results

  5. pET29a-YS14

    Plasmid
    #66890
    Purpose
    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)
    Depositor
    Jung-Hyun Min
    Article
    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.
    Inserts
    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    Tags
    H2A, H2B, H3, H4, His6 tag, and Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66890-plasmid-map-sequence-id-305064

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  14. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Article
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available Since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

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  23. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  36. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

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Showing: 5801 - 5840 of 10761 results