We narrowed to 26,494 results for: RON

1. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

   Plasmid

   #113696

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

   Plasmid

   #113695

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Adeno BN

   Plasmid

   #101823

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1

   Depositor

   Andrea Ventura

   Article

   Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Available Since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pmirGlo-CALB2-3'UTR delta ARE&mir30

   Plasmid

   #74430

   Purpose

   Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   CALB2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

   Plasmid

   #113691

   Purpose

   U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-EGFP-Tau

   Plasmid

   #46904

   Purpose

   expresses EGFP tagged Tau in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   MAPT (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   This WT htau construct contains human four-repeat…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. hGFAP-Cre

   Plasmid

   #40591

   Purpose

   Mouse astrocyte expression of Cre

   Depositor

   Albee Messing

   Article

   Zhuo et al Genesis. 2001 Oct;31(2):85-94.

   Insert

   Cre recombinase (GFAP Human, Mouse, Phage)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Mammalian

   Mutation

   Construct contains a nuclear-targeted Cre recombi…

   Promoter

   hGFAP promoter

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_human_ADAR2_ccB

    Plasmid

    #158113

    Purpose

    deletion hADAR2

    Depositor

    Emanuela Felley-Bosco

    Article

    Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

    Insert

    ADAR2 (ADARB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_human_ADAR2_ccA

    Plasmid

    #158114

    Purpose

    deletion hADAR2

    Depositor

    Emanuela Felley-Bosco

    Article

    Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

    Insert

    ADAR2 (ADARB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNoROS-CAT

    Plasmid

    #170152

    Purpose

    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

    Depositor

    Dimitri Pestov

    Article

    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

    Inserts

    catalase (Cat Mouse)
    roGFP2
    

    Use

    Genome integration via sleeping beauty transposon…

    Tags

    FLAG tag and Nucleolar Localization Sequence (NoL…

    Expression

    Mammalian

    Promoter

    RPBSA and TRE promoter

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB CAG SMARCA4-Venus

    Plasmid

    #153950

    Purpose

    piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

    Plasmid

    #113694

    Purpose

    U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187948

    Purpose

    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid

    #217897

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    

    Tags

    2xSTII

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9_human_ADAR2_ccC

    Plasmid

    #158112

    Purpose

    deletion hADAR2

    Depositor

    Emanuela Felley-Bosco

    Article

    Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

    Insert

    ADAR2 (ADARB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129028

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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