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Showing: 7351 - 7400 of 8515 results

  1. pMuLE Lenti Dest Neo

    Plasmid
    #62178
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62178-plasmid-map-sequence-id-101462

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  2. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Tags
    Expression
    Mutation
    Promoter
    C. glabrata MET3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

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  3. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Promoter
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  8. pFK210B-AtMIR390a-B/c

    Plasmid
    #51777
    Purpose
    Plant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51777-plasmid-map-sequence-id-79410

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  9. pMDC32B-AtTAS1c-B/c

    Plasmid
    #51773
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51773-plasmid-map-sequence-id-80112

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  13. pLyGo-Ec-7

    Plasmid
    #163135
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Nanobody, C-IgAP autotransporter
    Expression
    Bacterial
    Mutation
    Promoter
    P(rhaB)
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163135-plasmid-map-sequence-id-332144

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  14. pMDC123SB-AtTAS1c-B/c

    Plasmid
    #51772
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51772-plasmid-map-sequence-id-80110

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  15. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211845
    Purpose
    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Inserts
    LRR-NEL
    GAL4-VP16-GR
    Use
    Synthetic Biology
    Tags
    HA, Myc, and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    6xUAS-35Smini and pUBQ10
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    211845-plasmid-map-sequence-id-426288

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  18. pEMI91

    Plasmid
    #74888
    Purpose
    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information
    Depositor
    Piotr Marszalek
    Article
    Scholl et al Biomacromolecules. 2016 Jun 8.
    Insert
    Titin I27 x 9 (TTN Human)
    Use
    Tags
    His-tag and Strep-tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74888-plasmid-map-sequence-id-141569

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  19. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  21. MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid
    #176625
    Purpose
    TF A self-activation cassette in MultiFate-2.1
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    176625-plasmid-map-sequence-id-454352

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  24. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

    Plasmid
    #154829
    Purpose
    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    coEPO
    coEPO
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized (Cricetulus griseus)
    Promoter
    100RPU.2
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154829-plasmid-map-sequence-id-308567

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  26. pENTR-AtTAS1c-B/c

    Plasmid
    #51774
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Gateway-compatible entry clone
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51774-plasmid-map-sequence-id-79409

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  27. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104441-plasmid-map-sequence-id-201145

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  28. pEF004-sgRNA-shuffle-in

    Plasmid
    #104440
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    U6 promoter
    Available since
    Dec. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    104440-plasmid-map-sequence-id-201143

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  29. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

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  31. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

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  35. pSCL.180

    Plasmid
    #185001
    Purpose
    Express -Eco1 AAVS1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    AAVS1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185001-plasmid-map-sequence-id-376682

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  36. Th-tdTomato

    Plasmid
    #159460
    Purpose
    Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette
    Depositor
    Yuejun Chen
    Article
    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-
    Insert
    P2A-tdTomato
    Use
    Cre/Lox
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TH
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159460-plasmid-map-sequence-id-319267

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  37. pOCC119

    Plasmid
    #118884
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and mGFP, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118884-plasmid-map-sequence-id-232361

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  38. pCosSAD1AprR

    Plasmid
    #190786
    Purpose
    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination
    Depositor
    Jan Kormanec
    Article
    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(
    Inserts
    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    190786-plasmid-map-sequence-id-375582

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  41. pH7WG2B-OsMIR390-B/c

    Plasmid
    #61465
    Purpose
    Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Article
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    OsUBI
    Available since
    March 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    61465-plasmid-map-sequence-id-100075

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  43. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid
    #157983
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157983-plasmid-map-sequence-id-332422

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  44. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  46. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101725-plasmid-map-sequence-id-199888

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  47. AA427

    Plasmid
    #215961
    Purpose
    Fragmid fragment: (guide cassette) enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215961-plasmid-map-sequence-id-431415

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Showing: 7351 - 7400 of 8515 results