Addgene

We narrowed to 17,679 results for: Met

Showing: 701 - 750 of 17679 results

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pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1-M7A,H102A

Plasmid

#159168

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A,H102A

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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mito-yHS1-M7A,H102A

Plasmid

#159165

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A,H102A

Use

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_1

Plasmid

#138347

Purpose

Express guide RNA 1 for Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_2

Plasmid

#138348

Purpose

Express guide RNA 2 for mouse Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-K225insNM

Plasmid

#31974

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

inserted amino acids Asn-Met before Lys225

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pMBS-1125

Plasmid

#217999

Purpose

Start-methionine

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Start-Met

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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289aa ELL2

Plasmid

#127266

Purpose

For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Three Mets (133, 138, 186) to Ileu. Contains 289…

Promoter

T7

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-Rluc8

Plasmid

#79844

Purpose

Rluc8 reporter plasmid

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

Rluc8

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJC53.2/PRMT5

Plasmid

#35692

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 5

Use

Tags

Expression

Mutation

Promoter

Sp6 for sense, T7 for sense and antisense

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCAH01

Plasmid

#128162

Purpose

IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

Depositor

Michael Guarnieri, Calvin Henard

Article

Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKA-141

Plasmid

#79832

Purpose

Expresses RpBphP1-mCherry

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

BphP1-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#19002

Depositor

Jay Keasling

Article

Smolke et al Metab Eng. 2001 Oct . 3(4):313-21.

Insert

crtE, crtB

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0882

Plasmid

#11417

Depositor

Sung-Hou Kim

Article

Huang et al J Struct Funct Genomics. 2002 . 2(3):12

Insert

methyltransferase (MJ_0882 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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p126

Plasmid

#186176

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 126

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p146

Plasmid

#186178

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 146

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p156

Plasmid

#186179

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 156

Use

Synthetic Biology and Unspecified; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p136

Plasmid

#186177

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 136

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p166

Plasmid

#186180

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 166

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p246

Plasmid

#186183

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 246

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p226

Plasmid

#186181

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 226

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p236

Plasmid

#186182

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 236

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p266

Plasmid

#186185

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 266

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FRP1671

Plasmid

#64538

Purpose

Inducible MET15 gene

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

MET15 (MET17 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

(lexA-box)_4-P_CYC1_minimal

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJC53.2/Smed001246

Plasmid

#35693

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 1 homolog

Use

Tags

Expression

Mutation

Promoter

Sp6 for sense, T7 for sense and antisense

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only
pJC53.2/Smed005105

Plasmid

#35694

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 1 homolog

Use

Tags

Expression

Mutation

Promoter

Sp6 for sense, T7 for sense and antisense

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only
pKA-142

Plasmid

#79835

Purpose

Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

PpsR2-mVenus-CAAX

Use

Tags

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMVd1

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-160

Plasmid

#79840

Purpose

Expresses NLS-PpsR2-mVenus under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-mVenus

Use

Tags

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLys-M1 backbone

Plasmid

#109386

Purpose

Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GAG-Pan

Plasmid

#99640

Purpose

EGFP-mCherry Met71GAG reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Use

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAG (Glu)

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GAA-Pan

Plasmid

#99639

Purpose

EGFP-mCherry Met71GAA reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Use

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAA (Glu)

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-eGFP

Plasmid

#162823

Purpose

Doxycycline-inducible overexpression of eGFP

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V163A

Promoter

Tet ON

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
RNA motif plasmid EDEN15

Plasmid

#107252

Purpose

EDEN15 RNA motif

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

EDEN15 motif

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PHBA02

Plasmid

#83536

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t

Use

Tags

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPHT-PKA

Plasmid

#60936

Purpose

Fluorescent FPX sensor for cAMP

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PKA FPX Sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Use

Tags

Expression

Mutation

Promoter

PhmtB

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E.coli RaPID plasmid

Plasmid

#107251

Purpose

Expresses the E.coli RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

E.coli BirA*

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pD864-LacZ

Plasmid

#109380

Purpose

Rhamnose inducible LacZ expression. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pRHAM-LacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/SVNeo-103

Plasmid

#79841

Purpose

Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

RpBphP1-mCherry-Intersectin1 DHPH domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Use

Tags

6 His tag

Expression

Bacterial

Mutation

Promoter

ARA promoter

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pKA-144

Plasmid

#79839

Purpose

Expresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

RpBphP1-mCherry-Intersectin1 DHPH domain

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6_Ptac_BmHps_BmPhi_BsMdh

Plasmid

#129103

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Article

Whitaker et al Metab Eng. 2017 Jan;39:49-59. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)

Plasmid

#174527

Purpose

Dual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pD864-LacZ backbone

Plasmid

#109384

Purpose

Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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