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We narrowed to 1,583 results for: pbluescript

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Showing: 701 - 750 of 1583 results

  4. pInt-ERCreER

    Plasmid
    #190040
    Purpose
    This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).
    Depositor
    Kayoko Tanaka
    Article
    Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408
    Insert
    ERT2-Cre-ERT2 flanked by AAVS1
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    190040-plasmid-map-sequence-id-419230

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  6. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid
    #197264
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197264-plasmid-map-sequence-id-389660

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  9. pBS-3xpA

    Plasmid
    #176505
    Purpose
    3x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    3x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176505-plasmid-map-sequence-id-346556

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  10. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid
    #173674
    Purpose
    To clone and express a gRNA in Anopheles
    Depositor
    Eric Marois
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173674-plasmid-map-sequence-id-342962

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  12. pBS-ACTB-C200-GFPfr2

    Plasmid
    #170726
    Purpose
    HR donor vector for ACTB gene with the revised marker sequence for ASHRA.
    Depositor
    Natsuko Chiba
    Insert
    Donor sequence to ACTB gene for HR with the revised marker sequence
    Use
    Hr donor vector for human actb gene.
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170726-plasmid-map-sequence-id-343481

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  13. lox2272 Neo* lox2272

    Plasmid
    #168769
    Purpose
    lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168769-plasmid-map-sequence-id-337468

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  17. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid
    #127510
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127510-plasmid-map-sequence-id-250757

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  20. pPEE17

    Plasmid
    #128361
    Purpose
    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Promoter
    TEF1
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128361-plasmid-map-sequence-id-267942

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  23. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid
    #101347
    Purpose
    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Mutation
    delta Tat
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101347-plasmid-map-sequence-id-194257

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  28. pBS-AMVpl-γWT-nAchR

    Plasmid
    #97187
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97187-plasmid-map-sequence-id-183032

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  32. pBS SK(+)/mGATA2

    Plasmid
    #83823
    Purpose
    mGATA2 cDNA
    Depositor
    Douglas Engel
    Insert
    GATA2 (Gata2 Mouse)
    Expression
    Bacterial
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  40. SK-hPar3

    Plasmid
    #53229
    Purpose
    bacterial expression of human Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2RL2 Human)
    Expression
    Bacterial
    Available Since
    July 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. pJH131

    Plasmid
    #48260
    Purpose
    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    GAL10/GAL1 bidirectional
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    48260-plasmid-map-sequence-id-69695

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  50. pHyVec7

    Plasmid
    #34793
    Depositor
    Rob Steele
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    GFP, RFP, and intergenic region between Hydra rep…
    Promoter
    Hydra vulgaris actin promoter
    Available Since
    Jan. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    34793-plasmid-map-sequence-id-41832

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Showing: 701 - 750 of 1583 results