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pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-L
Plasmid#127477Purposecoding sequence for synthetic zinc finger ZF-ADD-LDepositorInsertZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
UseCell-free transcription–translationTagsPDZ cognate peptide ligand (VKESLV)ExpressionMutationPromoterT7Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS06
Plasmid#52647Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS06
UseTagsExpressionMammalianMutationM374Q mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS14
Plasmid#52651Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS14
UseTagsExpressionMammalianMutationdeleted G47 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET3a pro-hRegIIIa
Plasmid#64935Purposebacterial expression of full length human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
UseTagsExpressionBacterialMutationmutations made in 5' end to relax secondary …PromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
MolBC
Plasmid#92210PurposeExpression of H. influenzae MolBC (ABC Transporter) in E. coliDepositorUseTags8x Histidine Tag and Enterokinase Cleavage Site b…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCellFree_G03 TCF3
Plasmid#67110PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF3 (TCF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationTruncated isoformPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Oncostatin M toehold switch sensor
Plasmid#111909PurposeToehold switch sensor to detect Oncostatin M mRNA with GFP outputDepositorInsertOncostatin M toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Xlim-1 (Lhx1) XH32-3
Plasmid#74135PurposeBasic cDNA clone for the Xlim-1/Lhx1 gene. In Bluescript BS KS+. To prepare RNA for Sense: BamHI/T3; for antisense: XhoI/T7DepositorInsertlhx1 (lhx1 Frog)
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pNH-TrxT-S15
Plasmid#127822PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Park2_L (TAL 3012)
Plasmid#36004DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
Park2_R (TAL 3013)
Plasmid#36005DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S15
Plasmid#127823PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0006
Plasmid#42246PurposegRNA targeted to zebrafish gene rgs4DepositorInsertgRNA-rgs4 (rgs4 Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pK RagA-RagC(Q120L)
Plasmid#99701PurposeBacterial expression of RagA-RagC(Q120L)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mP14H1
Plasmid#84369Purposeantigen for mouse PRDM14 purification ( aa 1-231)DepositorInsertPRDM14
UseTags6xHisExpressionBacterialMutationonly aa 1-231 presentPromoterT7Available sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Fast-GCaMP-RS12
Plasmid#52650Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS12
UseTagsExpressionMammalianMutationdeleted R40/R41/K42 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptp4a3_L (TAL 3000)
Plasmid#35992DepositorInsertTAL array targeting ptp4a3 (ptp4a3a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL14 - Prp8 2142–2398
Plasmid#90264Purposeprotein expressionDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsN-terminal 6xHis tagExpressionBacterialMutationonly Jab1 domain (aa 2143-2398)PromoterT7Available sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 AND input A
Plasmid#107362PurposeInput A for pNP1 AND gate sfGFPDepositorInsertAND gate input A
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 AND input B
Plasmid#107363PurposeInput B for pNP1 AND gate sfGFPDepositorInsertAND gate input B
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only
