164,247 results

1. pDEST14-Cys-DogCatcher

   Plasmid

   #171929

   Purpose

   Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   Cys-DogCatcher
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EFS-LSL-nlsGFP-shRNA

   Plasmid

   #175584

   Purpose

   Conditional express shRNA with control of LoxP

   Depositor

   Christian Wolfrum

   Article

   Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

   Type

   Empty backbone

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

   Plasmid

   #188072

   Purpose

   Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

   Depositor

   Kristen Verhey

   Article

   Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

   Insert

   rat KIF5C (Kif5c Rat)
   

   Use

   Tags

   Flag and Halo

   Expression

   Mammalian

   Mutation

   aa1-560, deletion of aa2-6

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-RA

   Plasmid

   #209872

   Purpose

   Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Use

   Tags

   Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGAselect

   Plasmid

   #195714

   Purpose

   pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCrl

   Plasmid

   #122443

   Purpose

   Expresses entire mouse reelin ORF in mammalian cells

   Depositor

   Tom Curran

   Article

   D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

   Insert

   reelin (Reln Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_Gal4UAS_tBFP_PGK_mCitrine

   Plasmid

   #188386

   Purpose

   Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   Gal4UAS_tBFP_PGK_mCitrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-Sos1

   Plasmid

   #32920

   Depositor

   Dafna Bar-Sagi

   Article

   Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-BG4-NLS-mCer

    Plasmid

    #211448

    Purpose

    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

    Depositor

    Helmut Pospiech

    Insert

    Anti-DNA G-quadruplex single chain antibody BG4
    

    Use

    Tags

    NLS-mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PiggyBac-TetO-En1-t2a-Foxa2-hygro

    Plasmid

    #176483

    Purpose

    TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX_DSB_Spectrum-V3

    Plasmid

    #192476

    Purpose

    Lentiviral DSB Spectrum V3 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, mCherry, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0208

    Plasmid

    #142556

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOS005: superecliptic pHluorin (cytosolic)

    Plasmid

    #163049

    Purpose

    superecliptic pHluorin (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-VIVIT

    Plasmid

    #11106

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    VIVIT
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1749 (pDonor, R*MmeI)

    Plasmid

    #160733

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-V5-PTK2B

    Plasmid

    #127233

    Purpose

    Eukaryotic expression of PTK2B

    Depositor

    Kai Johnsson

    Article

    Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

    Insert

    PTK2B
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YFP-CL1

    Plasmid

    #11950

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    YFP-CL1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LD-B

    Plasmid

    #209861

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SspBmicro-mCh-p60

    Plasmid

    #190168

    Purpose

    Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Tags

    SspBmicro-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Tol2-BetaActin-SecA5-YFP

    Plasmid

    #105664

    Purpose

    Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

    Depositor

    Qing Deng

    Article

    Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

    Insert

    Secreted Annexin5-EYFP (ANXA5 Human)
    

    Use

    Tol2-gateway

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Beta-Actin

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-teLuc c-myc

    Plasmid

    #100026

    Purpose

    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    teLuc, a NanoLuc mutant
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    D19S, D85N, C164H

    Promoter

    CMV

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

    Plasmid

    #169325

    Purpose

    Expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Expression

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Col1a-frt-hygro-pA

    Plasmid

    #20730

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1A-frt-hygro-pA
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBHSTC104

    Plasmid

    #124356

    Purpose

    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 N-intein and C-intein (bicistronic)
    

    Use

    Tags

    CcdA-C (SFAD)

    Expression

    Bacterial

    Mutation

    Promoter

    STC

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pgRNA-modified

    Plasmid

    #84477

    Purpose

    guide RNA expression lentiviral construct

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    guide RNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. dPspCas13b-wtFTO

    Plasmid

    #223696

    Purpose

    Expressing fusion protein of dPspCas13b and wild type human FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    dPspCas13b-GGS-wtFTO (FTO Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSB700 lenti gRNA Blasto

    Plasmid

    #167904

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GZnP3 in pcDNA3.1+

    Plasmid

    #161738

    Purpose

    Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

    Depositor

    Yan Qin

    Article

    Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

    Insert

    GZnP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HA-TAZ

    Plasmid

    #32839

    Depositor

    Kunliang Guan

    Article

    Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLJM5-WT PHGDH

    Plasmid

    #83901

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EBFP2-H2B-6

    Plasmid

    #55243

    Purpose

    Localization: Nucleus/Histones, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge