We narrowed to 10,133 results for: gnas
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Plasmid#153497PurposeAspergillus awamori Glucoamylase Signal Peptide Donor PlasmidDepositorInsertGlucoamylase SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce- α Galactosidase SP
Plasmid#153504Purposeα Galactosidase Signal Peptide Donor PlasmidDepositorInsertα Galactosidase SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Gga-Lysozym SP
Plasmid#153505PurposeChicken Lysozym Signal Peptide Donor PlasmidDepositorInsertLysozym SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Prepro SP
Plasmid#153491PurposeS. cerevisiae Prepro Signal Peptide Donor PlasmidDepositorInsertPrepro SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Invertase 2 SP
Plasmid#153493PurposeS. cerevisiae Invertase Signal Peptide Donor PlasmidDepositorInsertInvertase 2 SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCAGGS)
Plasmid#118039PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
TagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe1889 HC_Kan_SV40-polyA_p11
Plasmid#100678Purposepart designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertSV40-polyA
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe1897 HC_Kan_C-KDEL_8a
Plasmid#100681Purposepart designed to occupy position 8a of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertC-KDEL
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe1903 HC_Kan_SV40-NLS_p6
Plasmid#100684Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertSV40-NLS
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe2030 HC_Kan_bGHpA_p16
Plasmid#100688Purposepart designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertbGHpA
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe2058 HC_Kan_Kozak_ATG_Palm_p6
Plasmid#100694Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak_ATG_Palm
UseSynthetic BiologyAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWp7-19
Plasmid#72751PurposepFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]DepositorInsertUBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]
UseLentiviralAvailable SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWp7-20
Plasmid#72752PurposepFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]DepositorInsertUBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]
UseLentiviralAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMPO 62
Plasmid#69870PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII- sspH2 signal peptide, M13 replicatDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMPO 59
Plasmid#68364PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII-sspH2 signal peptide, ColE1 replication originDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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mRFP-Lact-C2
Plasmid#74061PurposeFluorescent biosensor for phosphatidylserine.DepositorAvailable SinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
ExpressionYeastPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
TagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1-mitoRexYFP
Plasmid#60246PurposeMitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertmitoRexYFP
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDB364
Plasmid#110275PurposeN-terminal 10-His DsbC TEV-peptide fusionDepositorTypeEmpty backboneTags10-His, TEV cleavage peptide, and mature DsbC (i.…ExpressionBacterialPromoterT7Available SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL-sec
Plasmid#99845PurposeMammalian expression with secretion signal sequenceDepositorAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
TagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-NES-ZapCV2-Zinc dead
Plasmid#203850PurposeExpressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
TagsNuclear Export SignalExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee
Plasmid#140836Purposemammalian expression of SpiCee, a buffer of calcium that prevents downstream signalingDepositorInsertSpiCee
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCI-EGFP-NR1 wt
Plasmid#45446DepositorInsertNR1 (Grin1 Rat)
TagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-CTwinStrep
Plasmid#45941Purposeuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTagsPelB and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mScarlet-I
Plasmid#98818PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorInsertmts
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR2b wt
Plasmid#45447DepositorInsertNR2B (Grin2b Rat)
TagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR2a wt
Plasmid#45445DepositorInsertNR2A (Grin2a Rat)
TagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJEP205-AAV-TRE3G-GFP-pA
Plasmid#164789PurposeAAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+LuxG13
Plasmid#26161DepositorInsertBacterial luciferase operon + G13 promoter
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …Available SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-GPI
Plasmid#65294Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
TagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-mCherry-cNLS
Plasmid#138527PurposeInducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-tdTomato-cNLS
Plasmid#138529PurposeInducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-syn pA
Plasmid#99689PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniMutationdead Cas9Available SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neuronsDepositorInsertsmGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-2X snRP1
Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniMutationdead Cas9Available SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRB_SCF2_LD6_CTEV
Plasmid#58879PurposeMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseDepositorInsertMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
UseAAVExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVPromoterCytomegalo Virus(CMV)Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only