165,025 results

1. pTRK892

   Plasmid

   #71803

   Purpose

   Expression of mutated L. gasseri gusA from strong Ppgm promoter

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

   Insert

   gusA3
   

   Expression

   Bacterial

   Mutation

   D573A

   Promoter

   Ppgm

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV-hGFAP-GFP

   Plasmid

   #183906

   Purpose

   Expression of GFP under the human GFAP ABC1D promoter

   Depositor

   Chun-Li Zhang

   Article

   Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

   Insert

   GFP (GFAP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS415-GPD

   Plasmid

   #183940

   Purpose

   Control vector that has a GPD promoter with no insert

   Depositor

   Michael Downey

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-CmYLCV

   Plasmid

   #205245

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CmYLCV

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EGFR

   Plasmid

   #116731

   Purpose

   Lentiviral expression of EGFR

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-EGFP-GRAM-W

   Plasmid

   #211704

   Purpose

   Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1801_pmax-dCasRx

   Plasmid

   #118634

   Purpose

   Transient transfection; Expresses dCasRx; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   dCasRx
   

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A

   Promoter

   CAGGS

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. cEF.tk-GFP

   Plasmid

   #33308

   Depositor

   Linzhao Cheng

   Article

   Pomper et al Cell Res. 2009 Mar;19(3):370-9.

   Insert

   HSV-TK/GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP955

   Plasmid

   #98784

   Purpose

   Accessory Plasmid for PACE

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204341

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-i3/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROP_C9_Puro_PDGFRA

    Plasmid

    #183313

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRA
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGD248

    Plasmid

    #219498

    Purpose

    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_EGFR_p.L858R

    Plasmid

    #82906

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Mutation

    L858R

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available Since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mCherry-Cytb5

    Plasmid

    #182579

    Purpose

    Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Cytb5
    

    Tags

    Cytb5

    Expression

    Mammalian

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRL093 pattB_exorh:EGFP_MCS

    Plasmid

    #213514

    Purpose

    attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. nLbCas12a

    Plasmid

    #213745

    Purpose

    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    nLbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTRE-8XGTIIC-DsRED-DD

    Plasmid

    #115798

    Purpose

    lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP responsive promoter (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    DsRED-DHFR

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-dLight1.3b

    Plasmid

    #111056

    Purpose

    Expresses dLight1.3b in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3b
    

    Tags

    Flag tag

    Expression

    Mammalian

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

    Plasmid

    #187582

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF7 (SRSF7 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. Marathon-RT pentamutant for Split-PE (pEH107)

    Plasmid

    #190110

    Purpose

    Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, N26R, D74R, N116K, N197R

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pCAG-HA-rat Nrxn2beta AS4(-)

    Plasmid

    #58268

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn2beta AS4(-) (Nrxn2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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33. pCDZ2FE_Pogz_1-1410

    Plasmid

    #105674

    Purpose

    Expresses Flag-tagged full-length human POGZ protein

    Depositor

    Thanos Halazonetis

    Insert

    POGZ (POGZ Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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34. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

    Plasmid

    #36432

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Expression

    Insect

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMarC9-R6k

    Plasmid

    #89477

    Purpose

    transposon plasmid, compatible with Tn-Seq

    Depositor

    George Church

    Article

    Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

    Insert

    pMarC9-R6k
    

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP shRNA tet pLKO puro

    Plasmid

    #163017

    Purpose

    Control tet-inducible shRNA targeting eGFP

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF3054

    Plasmid

    #144530

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SALL4 (SALL4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lenti-25xAARE-minP-EGFP

    Plasmid

    #159666

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xAARE-minP-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Tags

    VNp6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge