We narrowed to 886 results for: fkbp

1. pBluescriptKS(-)-FRB

   Plasmid

   #104475

   Purpose

   Plasmid carrying FRB with C-terminal HA tag.

   Depositor

   Benjamin Glick

   Article

   Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

   Insert

   FRB (MTOR Human)
   

   Use

   Phagemid

   Tags

   HA tag

   Expression

   Mutation

   includes only the FRB domain

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEntry Tshld-GFP

   Plasmid

   #53211

   Purpose

   pEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.

   Depositor

   Matthew Pratt

   Article

   Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

   Insert

   Tshld-GFP
   

   Use

   Tags

   EGFP, Flag, and HA

   Expression

   Mutation

   Promoter

   none

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FRB-polySH33

   Plasmid

   #175233

   Purpose

   Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YIplac204-pOst1-ESCargo

   Plasmid

   #115420

   Purpose

   Expresses a regulatable secretory cargo for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBluescriptKS(-)-FRBx2

   Plasmid

   #104476

   Purpose

   Plasmid carrying FRBx2 with C-terminal HA tag.

   Depositor

   Benjamin Glick

   Article

   Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

   Insert

   FRBx2 (MTOR Human)
   

   Use

   Phagemid

   Tags

   HA tag

   Expression

   Mutation

   includes only the FRB domain

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YIplac204-pOst1-ESCargo*

   Plasmid

   #115427

   Purpose

   Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIplac128-pOst1-ESCargo

   Plasmid

   #115421

   Purpose

   Expresses a regulatable secretory cargo for yeast at the LEU2 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. XE240 Fz1-ER-GFP

   Plasmid

   #16815

   Depositor

   Randall Moon

   Article

   Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

   Insert

   Fz1-ER-GFP
   

   Use

   Xenopus expression

   Tags

   gfp

   Expression

   Mutation

   F36M in FKBP

   Promoter

   Available since

   May 19, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pERB174

   Plasmid

   #58291

   Purpose

   const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718

   Depositor

   Michael Lampson

   Article

   Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

   Inserts

   Mis12-GFP-FKBPx3
   inducible FRB-mCh
   FKBP-targeted miRNA
   FRB-mCh-Mad1^549-718
   

   Use

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pERB172

    Plasmid

    #58290

    Purpose

    const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    Mis12-GFP-FKBPx3
    inducible FRB-mCh
    FKBP-targeted miRNA
    FRB-mCh-Mad1^485-718-AAA
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. iXFGFR1k

    Plasmid

    #31259

    Depositor

    Masahiro Yamagishi

    Article

    Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

    Insert

    Xenopus FGFR1-ICD-FKBP (fgfr1-a Frog)
    

    Use

    Tags

    HA epitope tag and myristoylation signal

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pSkipFlox

    Plasmid

    #85797

    Purpose

    P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    split Cre recombinase
    

    Use

    Cre/Lox; P. falciparum expression

    Tags

    FKBP, FRB*

    Expression

    Mutation

    Promoter

    Pfcrt

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CF-PIPKi

    Plasmid

    #20158

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D253A mutation in PIPK. C-terminus (amino acids …

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUASt-ssBiP-ESCargo

    Plasmid

    #140166

    Purpose

    Expresses a regulatable secretory cargo for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pERB161

    Plasmid

    #58286

    Purpose

    const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    Mis12-GFP-FKBPx3
    inducible FRB-mCh-Mad1^485-584
    FKBP-targeted miRNA
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 485-584 of Mad1

    Promoter

    Available since

    Feb. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAGH51

    Plasmid

    #107255

    Purpose

    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    HU-FRB
    

    Use

    Synthetic Biology

    Tags

    FRB

    Expression

    Mutation

    Promoter

    native HU promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX-EF1a_NKX2-1_CdTag-HA

    Plasmid

    #221493

    Purpose

    NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-FRB-VMAc-CGFP

    Plasmid

    #70226

    Purpose

    Expresses C-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Ile 129 to Cys, Deleted amino acids 1-128

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAIP Nup62Fv2GFP

    Plasmid

    #139684

    Purpose

    Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

    Depositor

    Edward Campbell

    Article

    Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

    Insert

    Nup62 (NUP62 Human)
    

    Use

    Lentiviral

    Tags

    2 copies of GFP and Dimerization domain FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX Nup62Fv2GFP

    Plasmid

    #139683

    Purpose

    Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

    Depositor

    Edward Campbell

    Article

    Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

    Insert

    Nup62 (NUP62 Human)
    

    Use

    Lentiviral

    Tags

    2 copies of GFP and Dimerization domain FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YF-RhoA(CA)

    Plasmid

    #20153

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-RhoA(CA) (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTripz Casp1-IRES-mCherry

    Plasmid

    #187294

    Purpose

    This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

    Depositor

    Alpha Yap

    Article

    Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

    Insert

    Caspase1 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NCVB-ssGrl1-ESCargo*

    Plasmid

    #140167

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssGrl1-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    MTT1

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJin-290

    Plasmid

    #125718

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p3E-FRB-HA no-pA

    Plasmid

    #82594

    Purpose

    3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FRB-CFP-ras

    Plasmid

    #87451

    Purpose

    rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    CFP and ras fragment

    Expression

    Mammalian

    Mutation

    FKBP and rapamycin binding domain only

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMA3200

    Plasmid

    #46855

    Purpose

    A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    ExoY
    

    Use

    Lentiviral

    Tags

    FKBP E31G, R71G, K105E and Myc

    Expression

    Mutation

    Promoter

    TRE-Tight

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YIplac204-pOst1-ESCargo(Orange)

    Plasmid

    #140157

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YIplac128-pOst1-ESCargo(Orange)

    Plasmid

    #140158

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. YIplac128-pOst1-ESCargo(Crimson)

    Plasmid

    #140156

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMA3228

    Plasmid

    #46856

    Purpose

    A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    ExoY K81M
    

    Use

    Lentiviral

    Tags

    FKBP E31G, R71G, K105E and Myc

    Expression

    Mutation

    Promoter

    TRE-Tight

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMRX-IZ-SEC61B-mCherry-FRB

    Plasmid

    #233349

    Purpose

    Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    SEC61B (SEC61B Human)
    mCherry
    FRB
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T2098L mutant of FKBP-rapamycin binding domain of…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEF-pIgH-ESCargo(FTV)

    Plasmid

    #140160

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. YF-Rac1(CA)

    Plasmid

    #20150

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(CA) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    constitutively active mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. YF-Rac1(DN)

    Plasmid

    #20151

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(DN) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dominant negative mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pL106P-LUC-ENTR1A(w451-1)

    Plasmid

    #17455

    Purpose

    Gateway entry vector, Degron N-term Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    FKBP-Luciferase fusion (MTOR firefly)
    

    Use

    Luciferase; Entry vector

    Tags

    FKBP12DD

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pC4-RhE-FRB-Fis1

    Plasmid

    #68056

    Purpose

    express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

    Insert

    Fis1 (FIS1 Human)
    

    Use

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    Fis1 tail (aa 93-152)

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LAMP1-mCherry-FRB*

    Plasmid

    #207145

    Purpose

    Lysosome-targeted rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    FRB* (T2098L mutant of FKBP-rapamycin binding dom…

    Expression

    Mammalian

    Mutation

    Silent mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. FRB_nSuMMVp

    Plasmid

    #118970

    Purpose

    Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSuMMVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUSE-RapR-Src(KD)-myc

    Plasmid

    #25934

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-Src (Fkbp1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    This pUSE-RapR-Src(KD)-myc plasmid contains the k…

    Promoter

    Available since

    Oct. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. YF-Cdc42(CA)

    Plasmid

    #20152

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Cdc42(CA) (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cdc42H was inserted into the multiple cloning sit…

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Tom20-mCherry-GAI(1-92)

    Plasmid

    #37316

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    Tom20 and mCherry

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

48. FRB_nSbMVp

    Plasmid

    #118969

    Purpose

    Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSbMVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEGFP-RapR-FAK-YM

    Plasmid

    #25928

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pINT-PuroDDHNF4a2

    Plasmid

    #31443

    Depositor

    Gerhart Ryffel

    Article

    Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Phic31/attb

    Tags

    FKBP L106P

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge