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Addgene

We narrowed to 11,436 results for: 112

Showing: 8001 - 8050 of 11436 results
  1. pJK360

    Plasmid
    #71571
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)
    Depositor
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pJK385

    Plasmid
    #71572
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)
    Depositor
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pCMV-HA-mIFITM1-C49,50,83A

    Plasmid
    #58420
    Purpose
    Expresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)
    Depositor
    Insert
    IFITM1 (Ifitm1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Cysteines 49, 50, and 83 to Alanine
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pDD2013

    Plasmid
    #101364
    Purpose
    Name: pCBG68-K14ΔTATA. K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    Depositor
    Insert
    K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    Use
    Unspecified
    Tags
    Expression
    Mutation
    K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  5. pDD2022

    Plasmid
    #101366
    Purpose
    Name: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
    Depositor
    Insert
    RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
    Use
    Luciferase
    Tags
    Expression
    Mutation
    RBPjk binding site B mutation in pCBG68-LANApi (p…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  6. pDD2026

    Plasmid
    #101369
    Purpose
    Name: pCBG68-LANApiΔTATA. LANApi TATA box mutation in pCBG68-LANApi (pDD2002).
    Depositor
    Insert
    LANApi TATA box mutation in pCBG68-LANApi (pDD2002).
    Use
    Luciferase
    Tags
    Expression
    Mutation
    LANApi TATA box mutation in pCBG68-LANApi (pDD200…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  7. pDD2027

    Plasmid
    #101370
    Purpose
    Name: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).
    Depositor
    Insert
    LANApi TATA box mutation in pCBR-K14 (pDD2005).
    Use
    Luciferase
    Tags
    Expression
    Mutation
    LANApi TATA box mutation in pCBR-K14 (pDD2005).
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  8. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Insert
    yA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pUC57-mitoLbNOX

    Plasmid
    #74448
    Purpose
    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Insert
    mitoLbNOX
    Use
    Tags
    Flag tag with linker and mitochondrial targeting …
    Expression
    Mutation
    Human codon optimized
    Promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. UTS2R-DuET

    Plasmid
    #213386
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    UTS2R (UTS2R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8001 - 8050 of 11436 results